慢性萎缩性胃炎患者Runx3基因甲基化水平的研究  

Investigation on Methylation of Runx3 Gene in Patients with Chronic Atrophic Gastritis

作　　者：赵春娜[1] 肖丽丽[1] 王倍[1] 魏玥光 

机构地区：[1]大庆油田总医院消化内科,163000

出　　处：《胃肠病学》2016年第8期470-473,共4页Chinese Journal of Gastroenterology

摘　　要：背景:慢性萎缩性胃炎(CAG)是一种胃黏膜发生萎缩性改变的慢性胃炎,目前CAG外周血生物标记物的研究较少见。目的:探讨CAG患者外周血Runx3基因启动子区各CpG位点的甲基化水平。方法:选取2013年6月—2014年5月大庆油田总医院82例轻度CAG患者和73例中重度CAG患者,以45名胃黏膜正常者作为对照。采用MALDI-TOF-MS法测定Runx3基因启动子区各CpG位点的甲基化水平,荧光定量PCR法测定Runx3 mRNA表达,蛋白质印迹法测定Runx3蛋白表达。结果:与对照组和轻度CAG组相比,中重度CAG组Runx3基因启动子区CpG 13、CpG 14和CpG 15位点的甲基化水平明显升高(P<0.05);Runx3 mRNA和蛋白表达明显降低(P<0.05)。而轻度CAG组Runx3基因各CpG位点的甲基化水平、Runx3 mRNA和蛋白表达与对照组无明显差异(P>0.05)。结论:CAG患者外周血Runx3基因启动子区CpG 13、CpG 14和CpG 15位点的高甲基化可抑制Runx3表达,有望作为CAG临床分期的生物标记物。Background： Chronic atrophic gastritis（ CAG） is a kind of chronic gastritis with atrophic changes of gastric mucosa.The studies on peripheral blood biomarkers in CAG are rare. Aims： To investigate the methylation of peripheral blood CpG sites in Runx3 gene promoter region in CAG patients. Methods： Eighty-two mild CAG patients,73 moderate to severe CAG patients from June 2013 to May 2014 at Daqing Oilfield General Hospital were enrolled,and 45 patients with normal gastric mucosa were served as controls. The methylation of CpG sites in Runx3 gene promoter region was measured by MALDI-TOF-MS. mRNA expression of Runx3 was determined by fluorescent quantitative PCR,and the protein expression of Runx3 was determined by Western blotting. Results： Compared with the control group and mild CAG group,methylation levels of CpG 13,CpG 14 and CpG 15 sites in Runx3 gene promoter region were significantly increased in moderate to severe CAG group（ P〈0. 05）,the mRNA and protein expressions of Runx3 were significantly decreased（ P〈0. 05）. No significant differences in methylation of CpG sites in Runx3 gene promoter region and mRNA and protein expressions of Runx3 were found between mild CAG group and control group（ P〈0. 05）. Conclusions： The hypermethylation of peripheral blood CpG 13,CpG 14 and CpG 15 sites in Runx3 gene promoter region can inhibit the expression of Runx3 in CAG patients,and can be used potentially as the biomarker for clinical staging of CAG.

关 键 词：胃炎 萎缩性 RUNX3 甲基化 生物学标记 

分 类 号：R573.32[医药卫生—消化系统]