姜黄素对TNF-α诱导人结直肠癌细胞上皮间充质转化、迁移的影响  

Effect of curcumin on epithelial-mesenchymal transition and migration of colorectal cancer cells induced by TNF-α

作　　者：李倩[1] 徐春燕[1] 彭鹏[1] 覃蒙斌[1] 李素艳[1] 黄杰安[1] 

机构地区：[1]广西医科大学第一附属医院,南宁530021

出　　处：《山东医药》2016年第34期1-3,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81260365);广西中医药民族医药自筹经费科研课题项目(GZZC14-57)

摘　　要：目的观察姜黄素对肿瘤坏死因子-α(TNF-α)诱导结直肠癌细胞上皮间充质转化(EMT)的影响,并探讨其可能的机制。方法将人结直肠癌HCT116细胞分为3组,A、B组分别以TNF-α20 ng/m L、TNF-α20 ng/m L+姜黄素25μmol/L干预48 h,C组不干预。采用相差显微镜观察细胞形态变化,划痕实验观察细胞迁移能力,Western blot法检测EMT标记物E-钙黏蛋白(E-cadherin)、波形蛋白(Vimentin)及NF-κB信号通路的p65蛋白。结果 A组细胞多呈具有极性的纺锤状、长梭形,排列疏松,大部分出现细长伪足;B组细长伪足细胞较A组少见,凋落细胞明显多于A组;C组细胞大多数为多边形,细胞间存在一定的间隙,少量细胞出现短粗伪足。A、B、C组细胞迁移距离分别为(319.84±20.93)、(90.70±7.25)、(144.07±11.31)μm,P均<0.05。与C组比较,A组细胞E-cadherin表达减少,Vimentin、p65蛋白表达增加(P均<0.05或0.01);而B组较A组E-cadherin表达增加,Vimentin、p65蛋白表达减少(P均<0.05或0.01)。结论姜黄素可能通过NF-κB信号通路来抑制TNF-α诱导结直肠癌HCT116细胞的EMT发生、侵袭和转移。Objective To investigate the effect of curcumin on tumor necrosis factor-α（ TNF-α）-induced epithelialmesenchymal transition（ EMT） of colorectal cancer HCT116 cells and the possible mechanism. Methods The human colorectal cancer HCT116 cells were divided into three groups. Group A and group B were treated with 20 ng / m L TNF-α,20 ng /m L TNF-α ＋ 25 μmol / L curcumin for 48 h,respectively,and group C with routine culture. Using phase contrast microscope to observe the changes in cell morphology,cell scratch experiments to observed cell migration in each group. Western blotting was used to detect the expression levels of EMT markers E-cadherin,Vimentin and NF-κB signaling pathway-related P65 protein. Results Cells of group A were mostly spindle-shaped,fusiform and loosely arranged and most appeared elongated pseudopodia; in the group B,the slender pseudopodia cells were less,and apoptotic cells were more than those in the group A; most of cells in the group C were polygon,there was a little space between cells,and a few cells appeared stubby pseudopodia. The cell migration distance of groups A,B and C were（ 319. 84 ± 20. 93） μm,（ 90. 70 ± 7. 25） μm,（ 144. 07 ±11. 31） μm,respectively,and significant difference was found between these three groups（ all P 0. 05）. Compared with group C,the expression of E-cadherin in cells of group A was decreased,however,the expression of Vimentin and p65 protein was increased; and compared with group A,the expression of E-cadherin in cells of group B was increased,the expression of Vimentin and p65 protein was reduced. Conclusion Curcumin inhibited the occurrence,development and metastasis of TNF-α-induced EMT in HCT116 colorectal cancer cells probably through NF-κB signaling pathway.

关 键 词：结直肠癌 HCT116细胞 姜黄素 上皮间质转化 核转录因子ΚB 肿瘤坏死因子 

分 类 号：R735.37[医药卫生—肿瘤]