1,25-(OH)_2VitD_3对人胰腺癌细胞侵袭迁移能力及MMP-2、9、14表达的影响  

Effect of 1,25(OH)_2D_3 on migratory,invasive ability and expression of MMP-2,9 and 14 of human pancreatic cancer cell line PANC-1

作　　者：杨晟[1] 刘声财 陈文华[1] 胡腾腾 林军[1] 

机构地区：[1]武汉大学中南医院,武汉430071

出　　处：《山东医药》2016年第34期17-19,共3页Shandong Medical Journal

基　　金：湖北省自然科学基金面上项目(2014CFB747)

摘　　要：目的观察1,25-二羟基维生素D3[1,25-(OH)_2VitD_3]对人胰腺癌PANC-1细胞侵袭迁移能力的影响,以及基质金属蛋白酶(MMP)-2、9、14的表达变化。方法将不同浓度(0、25、50、75、100μmol/L)的1,25-(OH)_2VitD_3作用于PANC-1细胞,干预24 h时分别用Transwell细胞侵袭、迁移实验观察细胞的侵袭和迁移能力,干预48 h时用Western blot法检测细胞MMP-2、9、14蛋白。结果 0、25、50、75、100μmol/L的1,25-(OH)_2VitD_3干预PANC-1细胞24 h时,侵袭细胞数分别为(46.20±0.84)、(30.80±2.28)、(18.80±1.64)、(13.20±2.39)、(5.20±1.30)个/HP,迁移细胞数分别为(66.03±1.25)、(55.82±1.65)、(41.43±1.51)、(19.86±1.45)、(10.23±1.95)个/HP;随着1,25-(OH)_2VitD_3浓度增加侵袭及迁移细胞数逐渐减少,差异有统计学意义(P均<0.05)。1,25-(OH)_2VitD_3干预PANC-1细胞48 h后,随着1,25-(OH)_2VitD_3浓度增加PANC-1细胞MMP-2、9、14蛋白相对表达量逐渐减少,差异有统计学意义(P均<0.05)。结论 1,25-(OH)_2VitD_3可通过下调MMP-2、9、14蛋白的表达,从而抑制胰腺癌PANC-1细胞的侵袭与迁移。Objective To investigate the effect of 1,25-dihydroxyvitamin D_3[1,25（OH）_2D_3]on invasive and migratory abilities of human pancreatic cancer cell line PANC-1 and the changes in the expression of matrix metalloproteinases（ MMP）-2,9 and 14. Methods The routinely cultured human pancreatic cancer cell line PANC-1 in vitro was treated with different concentrations of 1,25（ OH）_2D_3（ 0,25,50,75 and 100 μmol/L）. After 24 hours,the abilities of migration and invasion in vitro in each group were detected by Transwell migration and invasion assays. After 48 hours,the protein expression of MMP-2,9 and 14 in each group was measured by Western blotting. Results After intervention for 24 h with different concentrations of 1,25（ OH）_2D_3（ 0,25,50,75 and 100 μmol/L）,every field＇s invasive cells in each group were（ 46. 20 ± 0. 84）,（ 30. 80 ± 2. 28）,（ 18. 80 ± 1. 64）,（ 13. 20 ± 2. 39） and（ 5. 20 ± 1. 30） / HP; and the migratory cells in each group were（ 66. 03 ± 1. 25）,（ 55. 82 ± 1. 65）,（ 41. 43 ± 1. 51）,（ 19. 86 ± 1. 45） and（ 10. 23 ± 1. 95） /HP,respectively. The migratory and invasive cells were decreased with the increase of concentration,and the difference was statistically significant（ all P 0. 05）. After intervention for 48 h with different concentrations of 1,25（ OH）2D_3,MMP-2,9 and 14 protein expression showed a declining trend with the increase of concentration,and the difference was statistically significant（ all P 0. 05）. Conclusion 1,25（ OH）_2D_3 can inhibit the invasion and migration of PANC-1cells through down-regulating the expression of MMP-2,9 and 14.

关 键 词：胰腺癌 细胞侵袭 细胞迁移 1 25-二羟基维生素D3 基质金属蛋白酶 

分 类 号：R735.9[医药卫生—肿瘤]