CD4^+CD25^+Foxp3^+调节性T细胞对结直肠癌肿瘤免疫的影响及与临床病理的关系  

CD4^+CD25^+Foxp3^+ regulatory T cells about how to inhibite the tumor immunity in colorectal cancer

作　　者：张爱军 严姣华 陶京[3] 赵建国[4] 焉正庆 熊雄 

机构地区：[1]湖北省武汉市武昌医院外一科,湖北武汉430063 [2]湖北省武汉市武昌医院质管办,湖北武汉430063 [3]华中科技大学同济医学院附属协和医院胰腺外科中心,湖北武汉430022 [4]华中科技大学同济医学院附属协和医院胃肠外科,湖北武汉430022

出　　处：《中国现代普通外科进展》2016年第7期505-510,共6页Chinese Journal of Current Advances in General Surgery

基　　金：武汉市卫计生委2015年度武汉市临床医学科研项目(wx15c18)

摘　　要：目的:初步研究CD4^+CD25^+Foxp3^+调节性T细胞在结直肠癌中抑制肿瘤免疫的可能机制。方法:结直肠癌组织48例,大肠息肉组织22例,正常大肠组织21例,采用免疫组化的方法检测Foxp3+调节性T细胞及IL-10阳性细胞在上述组织中的表达;采用RT-PCR的方法检测Foxp3及Stat3 m RNA在不同组织中的表达情况,用Pearson法分析Foxp3与IL-10、Stat3的相关性及其与肿瘤临床病理特点之间的关系。结果:Foxp3及IL-10在结直肠癌组织中阳性细胞表达率分别为45.8%、68.8%,较息肉组织(13.6%、31.8%)及正常组织(9.52%、23.8%)显著升高(P<0.01);肿瘤组织中Foxp3及Stat3m RNA的相对表达量分别为0.644±0.059、0.343±0.036,较息肉组织(0.322±0.020、0.212±0.028)及正常组织(0.309±0.016、0.202±0.021)明显增强(P<0.01);结直肠癌组织中Foxp3与Stat3基因表达量呈正相关(r=0.526.P<0.05),Foxp3与IL-10蛋白表达呈正相关(r=0.314,P<0.05);肿瘤组织中Foxp3的表达量与组织学分级、淋巴结转移、TNM分期相关(P<0.05),与年龄、性别无明显相关(P>0.05);Stat3的表达量与临床分期及淋巴结转移相关(P<0.05);IL-10表达量与肿瘤分化水平、临床分期相关(P<0.05)。结论:CD4+CD25+Foxp3+调节性T细胞通过Foxp3及其他相关抑制性细胞因子的表达抑制肿瘤免疫,进而引起肿瘤免疫逃逸,从而促进肿瘤的发生发展。Objective： To study the mechanism of CD4＋CD25＋Foxp3＋ regulatory T cells about how to inhibite the tumor immunity in colorectal cancer. Methods： Collect 32 cases of colorectal car- cinoma, 21 cases of polypus, 18 cases of normal tissues. The immunohistochemistry method was used to measure the number of Foxp3＋ regulatory T cells and IL-10 positive cells in above tissues and RT-PCR method was used to dectect the expression of Foxp3 and Stat3 mRNA in different tis- sues. Then analyze these indexes,s interaction with clinicopathological. Results： The expression rate of positive cells of Foxp3 and tL-10 in colorectal cancer was respectively 45,8% and 68.8%.1t was significantly higher than the potypus tissues（13.6%, 31 .8%）and normal tissues（9.52%, 23.8%） with a statistical difference （P〈0.01）; The relative expression amount of Foxp3 and Stat3 mRNA in tumor tissue was respectively 0.644 ± 0.059 and 0.343 ± 0.036. It was significantly increased com- pared with polypus （0.322 ± 0.020, 0.212 ± 0.028） and normal tissue （0.309 ± 0.016, 0.202± 0.021, P〈0.01 ）; Foxp3 mRNA expression in colorectat cancer tissues is positively correlated with Stat3（r=0. 526, P〈0.05）.Foxp3 protein expression is positively correlated with IL-10 （r=0.314, P〈0.05）. The expression of Foxp3 in cancer patients was visibley correlated with the histological grade, lymph node metastasis and TNM stage （P〈0.05）and there is no visible correlation with age and gender （P〉0.05）. The expression of IL-10 was correlated with the histological grade, TNM stage（P〈 0.05）. The expression of Stat3 was correlated with the lymph node metastasis, TNM stage,P〈0.05）. Con clusino： CD4＋CD25＋Foxp3＋ regulatory T cells inhibit tumor immunity and induce tumor immune es- cape through the expression of Foxp3 and some other related inhibitory cytokines and finally promote the tumor progression.

关 键 词：Treg·FO X P3·STA T3·结直肠癌·肿瘤免疫 

分 类 号：R735[医药卫生—肿瘤]