Bmi-1在口腔鳞癌组织中的表达及意义  

Bmi-1 Expression and its Clinical Significance in Oral Squamous Carcinoma Tissues

作　　者：刘庭庭[1] 马洪[1] 段晓峰[1] 

机构地区：[1]贵州医科大学口腔医学系,贵州贵阳550004

出　　处：《贵阳医学院学报》2016年第8期942-945,共4页Journal of Guiyang Medical College

基　　金：贵阳市科技局社会发展项目科研基金一项[筑科合同(2013103)]

摘　　要：目的:探讨Bmi-1基因在口腔鳞癌组织中的表达及意义。方法:收集口腔鳞癌(OSCC)患者手术切除新鲜标本30例作为OSCC组,癌旁0.5 cm外正常组织及正常体检者的口腔黏膜组织共30例作为正常对照组,免疫组织化学染色SP法检测两组组织中Bmi-1蛋白的表达,实时定量基因扩增荧光检测系统(QPCR)检测Bmi-1 mRNA的表达,比较不同临床理特征OSCC患者Bmi-1的表达差异。结果:OSCC组织中,Bmi-1蛋白和mRNA的表达明显高于正常对照组;在OSCC组织中,中低分化的OSCC组织的Bmi-1蛋白和mRNA表达高于高分化OSCC组织,差异具有统计学意义(P<0.05),Bmi-1表达与患者年龄、性别无关(P>0.05)。结论:Bmi-1基因检测可作为OSCC的辅助诊断指标之一。Objective： To explore the Bmi-1 gene expression and clinical significance in oral squa- mous carcinoma（OSCC） tissues. Methods： Fresh tissue samples of 30 patients with oral squamous cell carcinomas were collected as OSCC group, while 30 cases of 0.5 cm normal tissue adjacent to car- cinoma and normal physical examination were taken as the normal control group. The expression of Bmi-1 protein in the two groups was detected by immunohistochemistry SP method, and the expression of mRNA Bmi-1 was detected by real-time quantitative PCR. Results： The expression of Bmi-1 protein and mRNA in OSCC tissue were significantly higher than those in normal control group. In OSCC tis- sues, the expression of Bmi-1 protein and mRNA in the low and middle differentiated OSCC tissues were higher than those in the highly differentiated OSCC tissues, and the difference was statistically significant （ P 〈 0.05）. BMI-1 expression was not related to patients age and sex. Conclusion ： Broi-1 gene detection can be used as one of the auxiliary diagnostic indicators of oral squamous cell carcino mas.

关 键 词：BMI-1 基因 口腔肿瘤 癌 鳞状细胞 免疫组织化学 

分 类 号：R739.8[医药卫生—肿瘤]