供者淋巴细胞光化学疗法处理后预输注对小鼠心脏移植预后的影响  

Effects of Extracorporeal Photopheresis Treated Donor Lymphocyte Infusion on Graft Outcome After Murine Heart Transplantation

作　　者：叶俊生[1] 李娟[2] 周敏捷[1] 夏仁飞[1] 

机构地区：[1]南方医科大学南方医院肾移植科,广东广州510515 [2]南方医科大学护理学院,广东广州510515

出　　处：《医学临床研究》2016年第8期1470-1474,共5页Journal of Clinical Research

基　　金：广东省自然科学基金（2016A030313566）;南方医科大学南方医院院长基金（20152010）;广东省医学科研基金（A2015241）

摘　　要：[目的]探讨经体外光化学疗法（extracorporeal photopheresis，ECP）处理后的供者淋巴细胞预输注对小鼠心脏移植后移植物存活的影响。[方法]采用小鼠腹部异位心脏移植模型，供者为BALB／C小鼠，受者为C57BL／6小鼠。实验分为两组：对照组直接行心脏移植手术，ECP组于术前7d分离供者脾脏淋巴细胞，给予ECP处理后回输给受者，移植后观察移植物存活情况；部分受者于术后7d获取脾脏用于流式细胞术检测，移植心脏者行HE染色观察其组织学特点，并用免疫组化法检测组织T淋巴细胞浸润。[结果1ECP组移植心脏存活时间延长（20±4．67dVS7．5±0．70d，P〈0．001），伴有移植物排斥反应减轻，淋巴细胞浸润减少，以及外周效应性T细胞生成减少，CD4＋CD25＋goxp-3＋调节性T细胞增加。[结论]供者淋巴细胞ECP处理后预输注能延长小鼠移植心脏存活时间，其机制可能在于ECP诱导的凋亡淋巴细胞预输注减少了效应性T细胞的数量，并且增加了调节性T细胞的产生。[Objective] To investigate the effects of extracorporeal photopheresis（ECP） treated donor lym- phocyte infusion on graft survival after murine heart transplantation. [Methods]Abdominal ectopic heart trans- plantations were performed using BALB/C mice as donors and C57BL/6 mice as recipients. Mice in the control group did not receive any treatment other than transplantation. For mice in ECP treated group, donor spleno- cytes suspension was treated by ECP and then injected into recipients via penis vein 7 days before transplanta- tion. The graft survival was assessed by abdominal palpation after transplantation. The spleen were harvested on day 7 for further flow cytometry test; and the H＆E staining and immunohistochemistry staining of T lym- phocyte of graft tissue was evaluated. [Results]The graft survival time in the ECP treated group was signifi- cantly prolonged compared to the control group（20±4.67 d vs 7.5±0.70 d, P〈0.001）. The frequency of ef- fective T cells in the spleens of recipients in ECP treated group was significantly decreased compared to in the spleens of recipients in the control group. The frequency of CD4＋ CD25＋ Foxp-3＋ regulatory T cells in the spleen of ECP treated mice was higher than that of control group mice. [Conclusion]Infusion of ECP treated donor lymphocytes prolongs grab survival alter routine heart transplantation, possibly via suppressing effector T cell reaction and induction of regulatory T cell generation.

关 键 词：心脏移植 光化学疗法 移植物排斥 T淋巴细胞 调节性 预后 

分 类 号：R57[医药卫生—消化系统]