联合检测尿液NMP22与HA在膀胱癌诊断中的应用  

Joint Detection of Urinary NMP22 and HA in the Diagnosis of Bladder Cancer

作　　者：刘亦赤[1] 

机构地区：[1]福建省泉州市光前医院检验科,福建泉州362321

出　　处：《医学临床研究》2016年第8期1550-1551,1558,共3页Journal of Clinical Research

摘　　要：[目的]探讨尿液细胞核基质蛋白22（NMP22）和透明质酸（HA）联合检测在膀胱癌诊断中的意义。[方法]膀胱癌患者（A组）88例，膀胱炎患者（B组）132例、健康对照（C组）80例，收集其晨尿10mL，对尿液中NMP22定性检测，定量检测HA含量，比较HA、NMP22单独检测与两者联合检测对膀胱癌诊断的价值。[结果]A组、B组和C组尿液HA含量分别为（353．42±56．13）ng／mg，（254．12±40．19）ng／mg和（128．3±21．2）ng／mg，A组高于B组和C组（P〈0．05），B组高于C组（P〈0．05）；A，B，C组尿液NMP22阳性率分别为63．6％（56／88），11．4％（15／132）和10．0%（8／80），A组高于B组和C组（P〈0．05），B组与C组阳性率相比较差异无显著性（P〉0．05）。HA单独检测诊断膀胱癌的敏感性为88．6％（78／88），特异性为82．5％（175／212），准确度为84．3％（253／300）；NMP22的敏感性为64．2（56／88），特异性为89．2％（189／212），准确度为81．7％（245／300）；HA与NMP22联合检测诊断膀陇癌的敏感性为95．5％（84／88），特异性为96．7％（205／212），准确度为96．3％（289／300）；HA与NMPP22联合检测的敏感性、特异性和准确性均要高于HA和NMP22任一单独检测。[结论]联合检测尿液NMP22与HA要优于单独检测，可提高对膀胱癌的诊断效能。[Objective]To explore the significance of joint detection of the urinary nuclear matrix protein 22 （NMP22） and hyaluronic acid （HA） in the diagnosis of bladder cancer. [Methods]Observed were 88 con- firmed bladder cancer patients, 132 cases of cystitis patients, and 80 healthy people as the control group. All patientg morning urine of 10 ml was collected. A qualitative detection of NMP22 in the urine and quantitative detection of the amount of HA in the urine were performed. The diagnostic values of HA-alone detection, NMP22-alone detection, and joint detection of NMP22 and HA were observed and compared. [Results]The HA concentrations in the urine of the bladder cancer group （Group A）, the cystitis group （Group B）, and healthy group （Group C） respectively were （353.42±56.13 ） ng/mg, （254.12 ± 40.19 ） ng/mg, and （128.3± 21.2）ng/mg. Urine HA concentrations in bladder cancer group was higher than that of the cystitis group and healthy group （ P〈0.05）. Urine HA concentration of the cystitis group was higher than that of the healthy group （ P〈0.05）. The NMP22 positive rate of urine in the bladder cancer group, the cystitis group, and healthy group respectively were 63.6% （56/88）, 11.4% （15/132）, and 10.0% （8/80）. Urine NMP22 positive rate in the bladder cancer group was higher than that of cystitis group and healthy group （ P〈0.05）, but there was no significant difference between the bladder inflammation group and healthy control group （ P 0.05）. HA-alone detection diagnosis of bladder cancer had a sensitivity of 88.6 % （78/88）, specificity of 82.5 % （175/212）, and overall accuracy of 84.3% （253/300）. NMP22-alone detection diagnosis of bladder cancer had a sensitivity of 64.2% （56/88）, specificity of 89.2% （189/212）, and accuracy of 81.7% （245/300）. The joint detection of NMP22 and HA in the diagnosis of bladder cancer had a sensitivity of 95.5% （84/88）, specificity of 96.7%（205/212）, and accuracy of 96.3%（289/300）

关 键 词：核基质相关蛋白质类/尿 透明质酸/尿 膀胱肿瘤/诊断 

分 类 号：R737.14[医药卫生—肿瘤]