浑河底泥古菌群落结构季节性变化特征  

The temporal changes of archaeal community structure in the sediments of the Hun River

作　　者：石国强[1,2,3] 彭剑峰[1,2] 宋永会[1,2] 袁林江[3] 姜诗慧 王佳[1,2,3] 

机构地区：[1]中国环境科学研究院城市水环境科技创新基地,北京100012 [2]中国环境科学研究院环境基准与风险评估国家重点实验室,北京100012 [3]西安建筑科技大学环境与市政工程学院,西安710055

出　　处：《环境科学学报》2016年第9期3160-3168,共9页Acta Scientiae Circumstantiae

基　　金：国家水体污染控制与治理科技重大专项(No.2012ZX07202-005)~~

摘　　要：为探究不同水期河流底泥古菌群落结构特征,选取浑河底泥为研究对象,并于2014年4月(枯水期),9月(丰水期),11月(平水期)对浑河流域进行样品采样,通过PCR-DGGE技术获得3个水期古菌的DGGE指纹图谱,并进行数据分析.结果表明,平水期的古菌多样性和种群丰度都要好于枯水期和丰水期,多样性指数均高于2.10,种群丰度均高于0.94,且上游到下游均匀度波动平缓,数值维持在0.90左右,说明平水期浑河底泥古菌群落结构变化相对稳定.聚类分析结果表明,不同水期浑河底泥古菌群落结构多样性并没有明显的地区特征,但3个水期位于城市段的采样点的古菌亲缘性较为接近.冗余梯度分析(RDA)结果表明,枯水期时水中BOD5、p H和DO是影响古菌群落的主要因素;丰水期时水中的p H和NO-3-N是影响古菌群落的主要因素;平水期时水中的TP和NO-2-N是影响古菌群落的主要因素.To explore the characteristics of archaea community structure of river sediments within different water periods,PCR-DGGE was used to analyze the sediments from Hun River in dry season（ April）,wet season（ September） and normal season（ November）,respectively. DGGE fingerprints of archaea community in different seasons were obtained by the PCR-DGGE method and the Cluster Analysis method. The results show that the diversity of archaea community in normal season was richer than that in dry and wet seasons,Shannon-Wiener Index was higher than 2.10,and Richness Index was higher than 0.94. Evenness Index was about 0.90 and it varied gently from upstream to downstream,demonstrating that the structure variation of archaea communities in sediment of Hun River was stable. No distinct discrepancy of archaea community diversity in different regions was attained by Cluster Analysis,but archaea samples collected from urban points in different seasons were similar in affinity. The RDA results show that BOD5、p H and DO in dry season river were the principal factors influencing archaea community diversity,while in wet season,the p H and NO-3-N were the main factors,and in normal seasons,the TP and NO-2-N played key roles to influence the diversity of archaea community.

关 键 词：浑河 底泥 PCR-DGGE 古菌 群落结构 

分 类 号：X172[环境科学与工程—环境科学]