外周血Septin-9基因表达变化在评估卵巢上皮癌患者预后的意义  

Significance of expression changes of Septin-9 gene in peripheral blood in assessing prognosis of patients with epithelial ovarian cancer

作　　者：王甲涛 王雁冰[2] 王敏[3] 

机构地区：[1]山东省日照市妇幼保健院妇产科,山东日照276826 [2]山东省日照市人民医院,山东日照276826 [3]中国医科大学第四医院,辽宁沈阳110000

出　　处：《中国妇幼健康研究》2016年第8期930-932,共3页Chinese Journal of Woman and Child Health Research

基　　金：辽宁省自然科学基金资助项目2013021040

摘　　要：目的探讨卵巢上皮性癌患者外周血Septin-9基因表达变化及在评估患者预后中的意义。方法选取98例卵巢癌患者、27例卵巢交界性上皮性肿瘤、25例卵巢良性上皮肿瘤和50例健康体检妇女（健康对照组）,利用实时荧光定量PCR技术检测患者外周血Septin-9基因表达,利用受试者工作特征曲线（ROC曲线）法评估患者外周血Septin-9 mRNA表达量在诊断卵巢癌中的价值,以卵巢癌患者外周血Septin-9 mRNA表达量P25值作为界值,将患者分为高表达组（n=73）和低表达组（n=25）,利用Kaplan-Meier生存分析法分析患者外周血Septin-9 mRNA表达量对患者生存时间的影响。结果卵巢癌患者外周血Septin-9mRNA表达量（1.47±0.32）显著高于卵巢交界性上皮性肿瘤（1.21±0.25）、卵巢良性上皮肿瘤（1.23±0.27）和健康对照组（1.24±0.23）,差异有统计学意义（F=10.393,P〈0.001）;卵巢癌患者外周血Septin-9 mRNA表达量与淋巴结转移和远处转移有关（P〈0.05）;ROC曲线分析显示,外周血Septin-9 mRNA表达量在区别卵巢癌患者时曲线下面积0.705（95%CI：0.633~0.778）,当ROC曲线界值为1.35时,灵敏度为71.4%,特异度为62.7%;Kaplan-M eier生存分析显示,低表达组患者中位生存时间39.0个月,高表达组患者中位生存时间24.0个月,差异有统计学意义（χ2=9.636,P=0.002）。结论 Septin-9基因在卵巢癌患者外周血中呈高表达,可作为早期临床诊断及判定患者预后的潜在指标。Objective To investigate the expression changes of Septin-9 gene in peripheral blood of patients with epithelial ovarian cancer and their significance in assessing prognosis. Methods Ninety-eight cases of ovarian cancer,27 cases of borderline ovarian tumors,25 cases of benign epithelial ovarian cancer and 50 healthy women（ healthy control group） were selected. The expressions of Septin-9 gene in peripheral blood of patients were detected by using real-time fluorescence quantitative PCR. The value of the expressions of Septin-9 mRNA in peripheral blood of patients in the diagnosis of ovarian cancer was analyzed by using receiver operating characteristic curve（ ROC curve）. With the P25 value of the expression level of Septin-9 mRNA in peripheral blood of patients with ovarian cancer being the dividing value,the patients were divided into high expression group（ n = 73） and low expression group（ n = 25）. The effects of the expression of Septin-9 mRNA in peripheral blood of patients on their survival time were analyzed with Kaplan-Meier survival method. Results The expression level of Septin-9 mRNA in peripheral blood of patients with ovarian cancer was 1. 47 ± 0. 32,It was significantly higher than that of patients with borderline ovarian epithelial tumors,those with benign epithelial ovarian tumor and the healthy control group,which was1. 21 ± 0. 25,1. 23 ± 0. 27 and 1. 24 ± 0. 23,respectively. The differences were statistically significant（ F = 10. 939,P〈0. 001）. The expression level of Septin-9 mRNA in peripheral blood of patients with ovarian cancer was related to lymph node metastasis and distant metastasis（ P〈0. 05）. The ROC curve analysis showed that the area under the curve for the expression level of Septin-9 mRNA in peripheral blood in distinguishing ovarian cancer was 0. 705（ 95% CI： 0. 633- 0. 778）. When the dividing value of the ROC curve was1. 35,the sensitivity was 71. 4% and the specificity was 62. 7%. Kaplan-Meier survival analysis showed that the median survival

关 键 词：卵巢上皮性癌 外周血 Septin-9基因 预后 

分 类 号：R737.31[医药卫生—肿瘤]