葱兰叶挥发油成分分析及其对鲜牛奶的抑菌作用  

The analyses on chemical components in the leaf volatile oils of Zephyranthes candida(Lindl.)Herb.and their antimicrobial activities in the fresh milk

作　　者：卫强[1] 时雪风 

机构地区：[1]安徽新华学院药学院,安徽合肥230088

出　　处：《食品与机械》2016年第8期21-24,90,共5页Food and Machinery

基　　金：安徽省质量工程项目(编号:2014zy078)

摘　　要：采用水蒸气蒸馏（SDE）和超临界二氧化碳（sFBC（）z）提取叶中挥发油，以（七MS检测分析，考察挥发油在4℃、10d内对鲜牛奶致腐菌的抑菌效果。结果表明：两者不同成分有39种，其中SDE挥发油中主要有甲基环己烷、二十八烷、间二甲苯、邻苯二甲酸二丁酯；SFE-CO2挥发油主要有4-（4-乙基环己基）-1-戊基环己烯、甲苯、邻苯二甲酸二异辛酯、1乙氧基戊烷、4一乙烯基-2-甲氧基苯酚、2，4-二叔丁基苯酚。两者相同成分有16种，含量差异较大。SFE-CO2挥发油抑菌作用较强，MIC值范围为O．031～0．125mg／mL。SFE-C02挥发油成分为浓郁香气和更强的防腐效果提供了物质基础。Two kinds of volatile oil were extracted by steam distillation extraction （SDE） and supercritical fluid extraction of CO2 （SFE-CO2）, and then they were identified by gas chromatography-mass spectrometry （GC MS）. The antimicroble effects of the extract on different rot funguses in fresh milk was evaluated in 4℃ within 10 days. There were 39 different components in the two kinds of volatile oil, including methyl cyclohexane, octacosane, 1,3-dime-thyl-benzene, and dibutyl phthalate mainly found in the SDE volatile oil, and 4-（4-ethylcyclohexyl）-l-pentyl cyclohexene, toluene, 1,2- benzenedicar-boxylic acid, diisooctyl ester, 1-ethoxy-pentane, 2-methoxy-4 vinylphenol, and 2,4-bis（1,1-dimethylethyl） phenol mostly presented in the SFE-COz volatile oil. The differences among the contents of 20 common components in two oils was great. The SFE- CO2 volatile oil showed more effective antimicrobial activity with the MIC range of 0.031-0.125 mg/ml. Moreover, those from SFE-CO2 volatile oil coulld also provide better exotic perfume and stronger an- tiseptic functions.

关 键 词：葱兰叶 挥发油 鲜牛奶 抑菌活性 SDE SFE-CO2 

分 类 号：TS252.2[轻工技术与工程—农产品加工及贮藏工程]