3D Bioplotting of Gelatin/Alginate Scaffolds for Tissue Engineering:Influence of Crosslinking Degree and Pore Architecture on Physicochemical Properties  

作　　者：Ting Pan 

机构地区：[1]Department of Biomedical Engineering,School of Materials Science and Engineering,South China University of Technology,Guangzhou 510641,China [2]National Engineering Research Center for Tissue Restoration and Reconstruction,Guangzhou 5J0006,China [3]Guangdong Province Key Laboratory of Biomedical Engineering,,South China University of Technology,Guangzhou 510006,China

出　　处：《Journal of Materials Science & Technology》2016年第9期889-900,共12页材料科学技术（英文版）

基　　金：supported by the National Basic Research Program of China(“973 Program”,No.2012CB619100);the National Natural Science Foundation of China(Grant No.51372085);the Guangdong–Hongkong Common Technology Bidding Project(No.2013B010136003);the Postdoctoral Science Foundation of China(No.2013M542172)

摘　　要：Gelatin/Alginate hydrogels were engineered for bioplotting in tissue engineering. One major drawback of hydrogel scaffolds is the lack of adequate mechanical properties. In this study, using a bioplotter, we constructed the scaffolds with different pore architectures by deposition of gelatin/alginate hydrogels layerby-layer. The scaffolds with different crosslinking degree were obtained by post-crosslinking methods. Their physicochemical properties, as well as cell viability, were assessed. Different crosslinking methods had little influence on scaffold architecture, porosity, pore size and distribution. By contrast, the water absorption ability, degradation rate and mechanical properties of the scaffolds were dramatically affected by treatment with various concentrations of crosslinking agent （glutaraldehyde）. The crosslinking process using glutaraldehyde markedly improved the stability and mechanical strength of the hydrogel scaf- folds. Besides the post-processing methods, the pore architecture can also evidently affect the mechanical properties of the scaffolds. The crosslinked gelatin/alginate scaffolds showed a good potential to encap-sulate cells or drugs.Gelatin/Alginate hydrogels were engineered for bioplotting in tissue engineering. One major drawback of hydrogel scaffolds is the lack of adequate mechanical properties. In this study, using a bioplotter, we constructed the scaffolds with different pore architectures by deposition of gelatin/alginate hydrogels layerby-layer. The scaffolds with different crosslinking degree were obtained by post-crosslinking methods. Their physicochemical properties, as well as cell viability, were assessed. Different crosslinking methods had little influence on scaffold architecture, porosity, pore size and distribution. By contrast, the water absorption ability, degradation rate and mechanical properties of the scaffolds were dramatically affected by treatment with various concentrations of crosslinking agent （glutaraldehyde）. The crosslinking process using glutaraldehyde markedly improved the stability and mechanical strength of the hydrogel scaf- folds. Besides the post-processing methods, the pore architecture can also evidently affect the mechanical properties of the scaffolds. The crosslinked gelatin/alginate scaffolds showed a good potential to encap-sulate cells or drugs.

关 键 词：Bioplotting Tissue engineering Scaffolds Gelatin Alginate 

分 类 号：TS202.3[轻工技术与工程—食品科学] TB39[轻工技术与工程—食品科学与工程]