不同产地鸡眼草总黄酮含量及动态变化研究  

Total Flavonoid Analysis and Flavonoid Accumulation Dynamics in Kummerowia striata from Different Areas

作　　者：吴瑞云[1,2] 黄丽丹[2] 蒋婷[2] 梁宇萍 吴美琳[2] 刘虹[2] 陈龙清[1] 

机构地区：[1]华中农业大学园艺林学学院,园艺植物生物学教育部重点实验室,武汉430070 [2]中南民族大学生命科学学院,南方少数民族地区生物资源保护与综合利用工程中心,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2016年第3期51-53,61,共4页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家科学技术部科技基础性工作专项重点项目(2014FY110100);中南民族大学中央高校基本科研业务费专项资金项目(ZZY10008)

摘　　要：目的:对鸡眼草种质资源进行了总黄酮含量的测定和评价,并探讨了鸡眼草中总黄酮的动态变化规律.方法:超声波辅助提取总黄酮,以三乙胺为介质紫外分光光度法考察了鸡眼草的总黄酮含量.结果:不同产地鸡眼草总黄酮含量差异极显著,其中贵州凯里榕江鸡眼草为最高,贵州余庆龙溪鸡眼草含量最低;根、茎、叶的总黄酮含量差异极显著,由高到低为叶>茎>根;鸡眼草中总黄酮的积累高峰为6、7、8月;鸡眼草干燥低温中存放,总黄酮含量远高于常温保存.结论:鸡眼草叶片是合成和积累总黄酮的主要器官,夏季是采收鸡眼草的最佳时期并适宜低温干燥保存.鸡眼草总黄酮含量不仅与产地和采收时间有关,而且与生境也有关.Objective: To determinate and analyze the total flavonoids of Kummerowia striata. collected in different places,and to analyze the variety trends of total flavonoids in K. striata. Methods: Using spectrophotometry with the medium of triethylamine,the contents of total flavonoids were determined by ethanol solution extraction assisted with ultrasonic.Results: The contents of total flavonoids in K. striata from different producing areas were significantly different,which was the highest in K. striata from Rongjiang,Guizhou province,but the K. striata from Yuqing,the same province showed the lowest content. The total flavonoids among leaf blade,stem and root system were significantly different and the sequence from high to low was leaf blade > stem > root system. The peak time of total flavonoids accumulation was June,July and August. The total flavonoids of K. striata stored in low-temperature and dry places were much higher than that in usual storage. Conclusions: Leaf was the main organ for the biosynthesis and accumulation of total flavonoids in K. striata.Summer was the best time to harvest K. striata,and it is suitable to store at low-temperature and dry places. The results suggest that contents of total flavonoids relate not only to collecting place and collecting time,but also to plant biotope.

关 键 词：鸡眼草 总黄酮含量 产地 动态变化 

分 类 号：Q949.95[生物学—植物学]