延边黄牛体细胞核移植胚胎编码抗氧化酶的基因表达研究  

Expression of Genes Encoding Antioxidant Enzymes in Somatic Cell Nuclear Transfered Embryos of Yanbian Cattle

作　　者：杨月春[1,2] 王士勇[3] 李钟淑[2] 方南洙[2] 

机构地区：[1]吉林农业科技学院,吉林吉林132101 [2]延边大学农学院,吉林延吉133002 [3]中国农业科学院特产研究所,吉林长春130112

出　　处：《江西农业大学学报》2016年第4期706-710,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(30860184);吉林农业科技学院博士科研启动基金(吉农院合字2012305)~~

摘　　要：为研究延边黄牛体细胞核移植(SCNT)胚胎编码过氧化氢酶(CAT)、锰超氧化物酶(Mn-SOD)和谷胱甘肽过氧化物酶(GPx)的mRNA表达情况,以孤雌激活(PA)胚胎为对照,利用反转录PCR扩增延边黄牛SCNT胚胎编码CAT、Mn-SOD和GPx的mRNA,琼脂糖凝胶电泳后使用计算机软件进行半定量分析。结果显示:延边黄牛SCNT胚胎编码CAT的基因在2细胞期开始表达,且与PA胚胎存在显著差异(P<0.05);编码Mn-SOD的基因在8细胞期开始表达,与PA胚胎在16细胞期以上时期差异显著(P<0.05);编码GPx的基因在4细胞期开始表达,与PA胚胎在4细胞期差异显著(P<0.05),8细胞期以上时期差异不显著(P<0.05)。与孤雌激活胚胎的差异表明SCNT胚胎抗氧化酶表达的异常可能是由核移植操作过程所引起的。To study the mRNA expression of encoding catalase（ CAT）,superoxide enzyme manganese（ Mn-SOD） and glutathione peroxidase（ GPx） of Yanbian cattle somatic cell nuclear transfer（ SCNT） embryos,the CAT,Mn-SOD and GPx encoding mRNA in SCNT embryos were amplificated by reverse transcription PCR.The parthenogenetic activation（ PA） embryos were used as the control group. After agarose gel electrophoresis,semi-quantitative analysis was carried out using computer softwares.The results showed that the gene encoding CAT in SCNT embryos began to express in the 2-cell stage,and it was significantly different（ P〈0.05） from PA embryos; the gene encoding Mn-SOD began to express in 8-cell stage,and significantly different from PA embryos above the 16-cell period（ P〈0.05）; the gene encoding GPx began to express at the 4-cell stage,there was significant difference compared with PA 4-cell stage embryos（ P〈0.05）,however,no more difference was found after the 8-cell stage（ P〈0.05）.These results indicate that the abnormal expression of antioxidant enzymes may be caused by nuclear transplantation operation during SCNT.

关 键 词：延边黄牛 体细胞核移植 孤雌激活 抗氧化酶 基因表达 

分 类 号：S823.81[农业科学—畜牧学]