三正辛胺络合萃取法分离提取糖果中8种合成色素  

Separation and Extraction of Eight Synthetic Pigments in Candy by Complexing Extraction with Tri-n-octylamine

作　　者：欧阳丽[1] 黎瑛[1] 章建辉[1] 夏立新[1] 胡丽俐[1] 李莎[1] 

机构地区：[1]长沙市食品质量安全监督检测中心,湖南长沙410013

出　　处：《分析测试学报》2016年第9期1105-1110,共6页Journal of Instrumental Analysis

基　　金：国家公益性行业科研专项项目(201310130)

摘　　要：以三正辛胺为络合剂，四氯化碳为稀释剂，采用络合萃取法对糖果中的柠檬黄、新红、苋菜红、胭脂红、日落黄、诱惑红、亮蓝和赤藓红8种合成色素进行了预处理研究。考察了样品溶液pH值、稀释剂种类、三正辛胺浓度、反萃相组成等对预处理效果的影响。结果表明，当样品溶液pH值为6.0，以四氯化碳为稀释剂，三正辛胺浓度为5%，无水乙醇-15%氨水（体积比1∶1）为反萃相时，8种合成色素的回收率为90%~98%。该预处理方法操作简单、快速、有机溶剂用量少、回收率高，适用于糖果中多种合成色素的分离提取。Complexing extraction was applied in the pretreatment of eight synthetic pigments such as tartrazine,new red,amaranth,ponceau 4R,sunset yellow,allura red,brilliant blue and erythrosine in candy with tri-n-octylamine（TNOA） as complexing agent and tetrachloromethane as diluent. Effects of operation conditions such as pH of working solution,kind of diluent,TNOA concentration and the strip solution composition on pretreatment efficiency were investigated.Results showed that when pH value of working solution was 6.0,the diluent was tetrachloromethane,TNOA concentration was 5% and the strip solution was ethanol-15% ammonium solution（1∶1,by volume）,the recoveries of eight synthetic pigments were in the range of 90%-98%.The results indicated that the method showed the advantages of simplicity,rapidness,low solvent consumption and high recovery, and was suitable for the separation and extraction of synthetic pigments in candy.

关 键 词：三正辛胺 合成色素 络合萃取 高效液相色谱(HPLC) 

分 类 号：O657.72[理学—分析化学] TS264.4[理学—化学]