IGF-1R抑制剂NVP-AEW541在肾上腺皮质癌SW13细胞系中的作用  

The effect of IGF-1R inhibitor NVP-AEW541 on adrenocortical carcinoma SW13 cell line

作　　者：林登强 徐烈雨 廉建坡 王晓晶[1] 何竑超[1] 王卫庆[2] 宁光[2] 祝宇[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院泌尿外科,上海200025 [2]上海交通大学医学院附属瑞金医院内分泌科,上海200025

出　　处：《现代泌尿外科杂志》2016年第9期709-712,共4页Journal of Modern Urology

基　　金：国家自然科学基金(No.81272936);上海市科委医学引导项目基金(No.134119a2700)

摘　　要：目的通过细胞实验,探寻IGF-1R抑制剂NVP-AEW541阻断IGFR信号通路的激活,评价其对SW13细胞增殖、凋亡的影响情况。方法通过MTT试验观察细胞增殖,通过流式细胞仪检测细胞凋亡的改变,通过Western blot检测IGFR下游信号通路成员ERK和mTOR的表达情况。结果 NVP-AEW541可以呈浓度-时间依赖性抑制细胞增殖、呈浓度依赖性促进细胞早期凋亡和晚期坏死,并下调p-AKT的表达水平,而不影响mTOR的磷酸化水平和RAS/RAF/ERK信号传导通路。结论 IGF-1R抑制剂NVP-AEW541可以通过下调AKT的磷酸化水平,从而抑制细胞增殖、促进细胞凋亡,具有潜在的抗肿瘤作用。Objective To explore the effect of NVP-AEW541 on adrenocortical carcinoma（ACC） cell line SW13 in vitro. Methods After SW13 cells were treated with NVP-AEW541,the proliferation and apoptosis were detected with MTT assay and flow cytometryrespectively. The expressions of ERK and roTOR were detected with Western blotting. Results NVP- AEW541inhibitedtheproliferationin a dose- and time-dependent manner, promotedtheapoptosis in a dose-dependent manner, down-regulatedthelevel of p-AKT protein expression, but did not affect the expression of p-mTOR protein and RAS/RAF/ ERK signaling pathway. Conclusions NVP-AEW541 is effective to inhibit SW13 proliferation and promote SW13 apoptosis by reducing the expression level of p-AKT. It has anti-tumor potential.

关 键 词：SW13 NVP—AEW541 胰岛素样生长因子 PI3K信号通路 

分 类 号：R736.6[医药卫生—肿瘤]