猪氨肽酶N(APN)基因密码子偏好性分析  

Analysis of codon use features of APN gene in pigs

作　　者：宗秋芳 戴开宇[1] 刘颖[1] 吴正常[1] 吴圣龙[1] 包文斌[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2016年第2期45-50,73,共7页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：转基因生物新品种培育科技重大专项(2014ZX08006-001B);江苏省高等学校大学生创新创业训练计划项目(201511117030Z);扬州市国内产学研协同创新项目(2014-21);苏州市科技支撑计划项目(SNG201410)

摘　　要：为了解猪氨肽酶N(APN)基因的密码子使用特性,运用EMBOSS软件包分析猪APN基因的密码子偏好性,并与模式生物基因组及其他13个物种APN基因进行比较,为选择合适的受体动物及最佳外源表达系统提供指导和依据。结果表明:猪APN基因大部分偏好使用以G/C结尾的密码子,存在23种偏好密码子(RSCU>1),不同物种APN基因密码子偏好性比较发现14个物种的APN基因表达水平一般;基于RSCU和CDS的聚类分析发现,CDS的系统进化树更接近这14个物种的真实系统分类;在密码子的使用频率上,猪APN基因与小鼠基因组的差异小于大肠埃希菌和酵母菌等2种模式生物基因组。这一研究为APN基因在动物遗传改良中选择合适的受体动物、选择最佳的外源表达系统及提高其表达水平提供一定的理论依据。The objective of our study is to understand APN gene codon use features and provide guidance and basis for selecting appropriate expression systems. In this research, pig APN gene was analyzed by EMBOSS software package, and then compared with genomes in model organisms and APN genes from 14 animal species. The results showed that most of the pig APN genes were bias toward the synonymous codons with G and C at the third codon position. There were twenty-three biased codons （RSCU~ 1）. The comparative analysis of APN genes from different animal species showed that gene expression level was general in 14 animal species. RSCU and CDS clustering analysis showed that the phylogenetic tree based on CDS was closer to the real system classification of 14 species. The differences in codon usage frequency between pig APN gene and genome of Mus musculus were less than those in other two kinds of model organ- isms＇ genome. This study could provide certain theoretical basis for selecting the appropriate receptor species in animal genetic improvement, selecting appropriate expression systems and improving the expression level of APN gene.

关 键 词：猪 APN基因 密码子偏好性 

分 类 号：S828.2[农业科学—畜牧学]