气相色谱法和液相色谱-串联质谱法测定水产品中敌敌畏残留量  

Determination of dichlorvos residue in aquatic products by gas chromatography and liquid chromatography-tandem mass spectrometry

作　　者：吕磊[1] 彭婕[1] 何力[1] 甘金华[1] 喻亚丽[1] 

机构地区：[1]中国水产科学研究院长江水产研究所,农业部水产品质量安全风险评估实验室(武汉),武汉430223

出　　处：《分析试验室》2016年第9期1074-1077,共4页Chinese Journal of Analysis Laboratory

摘　　要：建立了水产品中敌敌畏残留量检测的两种不同方法,比较了气相色谱（GC）与液相色谱-串联质谱（LC-MS/MS）检测方法的适用性。GC法检出限为3.0μg/kg,定量限为10.0μg/kg;LC-MS/MS法检出限为0.3μg/kg,定量限为1.0μg/kg。当敌敌畏添加水平在5.0~15.0μg/kg时,GC法方法回收率为75.0%~90.9%,相对标准偏差为2.8%~9.6%;LC-MS/MS法方法回收率为89.7%~98.1%,相对标准偏差为1.0%~5.0%。结果表明,应用GC与LC-M S/M S检测方法均能满足水产品中敌敌畏残留分析要求,但在不考虑实验成本的前提下,LC-MS/MS法更具有优势。Two different methods were developed for the determination of dichlorvos residue in aquatic products by Gas Chromatography（GC） and Liquid Chromatography-Tandem Mass Spectrometry（LC-MS/MS）.Comparison of the applicability between two methods was carried out. The limit of detection（LOD） by GC was3. 0 μg/kg,and the limit of quantitation（LOQ） was 10. 0 μg/kg; the LOD by LC-MS/MS was 0. 3 μg/kg,and the LOQ was 1. 0 μg/kg. At the spiked levels of 5. 0 ~ 15. 0 μg/kg,the recoveries were 75. 0% ~ 90. 9%with the relative standard deviations（RSD） of 2. 8% ~ 9. 6% by GC; the recoveries were 89. 7% ~ 98. 1%with the RSD of 1. 0% ~ 5. 0% by LC-MS/MS. Both methods were suitable for the determination of dichlorvos residue in aquatic products. Without considering the experimental cost,the method by LC-MS/MS has more advantages.

关 键 词：气相色谱 液相色谱-串联质谱 敌敌畏 水产品 

分 类 号：TQ450.7[化学工程—农药化工]