酸碱预处理后酶解提升丹参药渣中丹参酮类成分的提取效率研究  

Effect of enzymolysis after acid and alkali pretreatment on extraction of tanshinones from Salviae Miltiorrhizae Radix et Rhizoma residues

作　　者：戴新新 沈飞[1] 宿树兰[1] 张森[1] 郭盛[1] 江曙[1] 钱大玮[1] 段金廒[1] 

机构地区：[1]南京中医药大学江苏省中药资源产业化过程协同创新中心/中药资源产业化与方剂创新药物国家地方联合工程研究中心,国家中医药管理局中药资源循环利用重点研究室,江苏南京210023

出　　处：《中国中药杂志》2016年第18期3355-3360,共6页China Journal of Chinese Materia Medica

基　　金：江苏高校中药资源产业化过程协同创新中心建设专项;国家科技支撑计划项目(2012BAI29B02);江苏省“333高层次人才工程”项目(2013,2015);江苏高校中药学优势学科Ⅱ期建设工程(2014-ysxk)

摘　　要：通过酸碱预处理丹参药渣,并利用纤维素酶降解,比较不同处理方法对丹参酮类成分提取效率的影响,以期为丹参药渣中丹参酮类成分的开发利用提供科学依据。结果表明,未经酸碱预处理的丹参药渣中,当酶C浓度为6 U·m L^(-1),酶解4.5 d时可使大部分纤维素降解,所得葡萄糖质量分数最高为59.74 mg·g^(-1)。对不同预处理方法评价,发现碱预处理-纤维素酶C降解后的效果最佳,葡萄糖质量分数达119.50 mg·g^(-1),相同浓度纤维素酶C酶解的酸预处理药渣次之。丹参酮的提取量经酶液降解后,与常规非酶法处理相比,丹参酮ⅡA提取量提高了82.54%,质量分数达2.451 mg·g^(-1);丹参酮Ⅰ提取量提高了81.82%,质量分数达2.373 mg·g^(-1);隐丹参酮提取量提高了64.4%,质量分数达1.080 mg·g^(-1);二氢丹参酮Ⅰ提取量提高了61.3%,质量分数达0.601 2 mg·g^(-1)。通过酸碱预处理与纤维素酶降解相结合的方法可有效提高丹参药渣中丹参酮类成分的提取效率,该方法具有可操作性和实用性,有利于提升丹参药渣中丹参酮类资源性化学物质的利用效率。Salviae Mihiorrhizae Radix et Rhizoma residues were pre-treated with acid and alkali, degraded by using cellulose, and the effects of different processing methods on the extraction rate of tanshinones were compared to provide scientific basis for development and utilization of tanshinones from Salviae Miltiorrhizae Radix et Rhizoma residues. The results showed that in the Salviae Mihiorrhizae Radix et Rhizoma residues without pre-treatment, enzymatic hydrolysis time of 4. 5 d could make most of the cellulose degraded when the concentration of substrate enzyme concentration was 6 U ~ mL- ~, and the highest glucose concentration was 59. 74 mg ~ g- 1. It was found that the best effect was achieved after alkali pre-treatment-cellulose C degradation among the different pre-treatment methods, and the glucose content reached 119. 50 mg g-1, followed by the same concentration of acid pre-treatment-cellulose C degradation. The extraction amount of tanshinone lI A was increased by 82. 54% after enzyme degradation, with a mass fraction of 2.451 mg g-1 ; ex- traction amount of tanshinone I was increased by 81.82% after enzyme degradation, with a mass fraction of 2. 373 mg g-1 ; extraction amount of cryptotanshinone was increased by 64. 4% after enzyme degradation, with a mass fraction of 1. 080 mg g ; extraction amount of dihydrotanshinone I was increased by 61.3% after enzyme degradation, with a mass fraction of 0. 60l 2 mg g-1 Acid and alkali pre-treatment combined with cellulose degradation could effectively improve the extraction rate of tanshinones from Salviae Milti- orrhizae Radix et Rhizoma residues. This method is operable and practical, and it is beneficial for improving the utilization efficiency of tanshinones （resource based chemicals） from Salviae MiltioiThizae Radix et Rhizoma residues.

关 键 词：丹参药渣 丹参酮 酸碱预处理 酶解 

分 类 号：R284.2[医药卫生—中药学]