MiR-124对鼻咽癌细胞株上皮间充质转化和放疗敏感性的影响  

Effect of miR-124 on the epithelial-mesenchymal transition and radio-resistance of nasopharyngeal carcinoma

作　　者：田允鸿[1] 曾星[1] 邱慧芝[1] 史建军[1] 谢国丰[1] 黄东兰[1] 王红梅[1] 郑荣辉[1] 张伟军[1] 

机构地区：[1]广州医科大学附属肿瘤医院放疗科一区,510095

出　　处：《实用医学杂志》2016年第17期2780-2783,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81502342);广州医科大学博士启动基金资助项目(编号:2014C45)

摘　　要：目的:研究miR-124对鼻咽癌细胞株上皮间充质转化(epithelial-mesenchymal transitions,EMT)和放疗敏感性的影响。方法:转染miR-124 mimic或inhibitor入鼻咽癌细胞株,划痕实验检测miR-124对鼻咽癌EMT影响;细胞转染成功24 h后X线照射,检测细胞株中Caspase-3活性及凋亡细胞比例;Ed U方法检测放疗后细胞增殖能力;Western blot检测信号通路蛋白。结果:划痕实验结果显示miR-124能抑制鼻咽癌细胞株EMT。Caspase-3活性检测结果提示,miR-124过表达细胞株接受放疗后Caspase-3活性显著上升(P<0.01)。流式细胞仪结果显示,miR-124能增加细胞株放疗后凋亡。Ed U结果显示,miR-124能抑制放疗后细胞的增殖。Western blot结果表明过表达miR-124的细胞株接受放疗后,p-Akt含量显著下降。结论:miR-124可能通过Akt信号通路抑制鼻咽癌细胞株EMT,增加其放疗敏感性。这为鼻咽放疗增敏提供了新的可行的研究方向。Objective To identify the role of miR-124 in regulating the radiosensitivity and the epithelial-mesenchymal transition （EMT） of nasopharyngeal carcinoma （NPC）. Methods Transient transfection of cells with miR-124 mimic or inhibitor was performed and wound-healing assay was used to investigate the role of miR-124 in the EMT of NPC. The apoptosis affected by miR-124 was also measured after irradiation, followed by investigating the cell proliferation by EdU assay. Finally, proteins of Akt and ERK associated with EMT and radiosensitivity, were measured by western blot. Results The migration index from NPC cell line indicated that miR-124 repressed the EMT. The results from caspase-3 activity assay showed that caspase-3 activity after irradiation significantly increased in miR-124 mimic group compared with the control group （P 〈 0.01）. It was also confirmed that irradiation led to a higher percentage of apoptosis in miR-124 group compared with the control group in NPC cells. Cell proliferation after irradiation was significantly decreased in MiR-124 group as compared with control group. MiR-124 inhibited the protein expression of p-Akt. Conclusion MiR-124 may repress the EMT and decrease radio-resistance of NPC via p-Akt signaling pathway, which may provide a new insight into radio-resistance in NPC.

关 键 词：MiR-124 鼻咽癌 放疗敏感性 上皮间充质转化 

分 类 号：R739.63[医药卫生—肿瘤]