EGFR抑制剂对舌鳞癌多药耐药细胞Tca8113/CBP耐药蛋白的影响  

The effect of anti-EGFR agents on multidrug resistance in tongue squamous cell carcinoma drug-resistance cell Tca8113/CBP

作　　者：刘得玺[1] 窦志茜 李娟[1] 张丽[1] 倪小兵[1] 牛玉明[1] 罗志晓[1] 冷卫东[1] 

机构地区：[1]湖北医药学院附属十堰市太和医院口腔医学中心,湖北十堰442000 [2]湖北医药学院附属十堰市太和医院产科,湖北十堰442000

出　　处：《临床口腔医学杂志》2016年第8期459-461,共3页Journal of Clinical Stomatology

基　　金：湖北省教育厅科研计划项目(D20122405)

摘　　要：目的:探讨表皮生长因子受体(EGFR)抑制剂在舌鳞癌多药耐药中的作用。方法:免疫细胞化学技术和Western blot方法检测舌癌细胞系Tca8113及多药耐药细胞系Tca8113/CBP EGFR的表达,Western blot检测EGFR受到抑制后舌癌多药耐药细胞Tca8113/CBP耐药蛋白MRP,GTS-π的表达。结果:细胞系Tca8113和Tca8113/CBP均有EGFR的表达(P>0.05),EGFR受到抑制后细胞Tca8113/CBP的多药耐药蛋白MRP,GST-π的表达均下降(P<0.05)。结论:抑制EGFR可下调舌癌多药耐药细胞耐药蛋白的表达。Objective：To study the effect of anti-EGFR agents on multidrug resistance（ MDR） in tongue squamous cell carcinoma drug-resistance cell line Tca8113/CBP.Methods：Immunohistochemical staining and western blot assay were applied to detect EGFR expression in tongue squamous cell carcinoma cell line Tca8113 and its MDR cell line Tca8113/CBP, the protein expression levels of MRP, GST-πin cell line Tca8113/CBP which EGFR expression were inhibited were detected by western blot assay.Results：We found higher expression of EGFR in cell lineTca8113 and its MDR cell line Tca8113/CBP（P〉0.05）.After the EGFR expression were inhibited,the expression MRP and GTS-πwere significant decrease in MDR cell line Tca8113/CBP（ P〈0.05） . Conclusion：The anti--EGFR agents can lead to a downregulation of resistance protein expression.

关 键 词：EGFR 西妥昔 舌癌 多药耐药 Tca8113/CBP细胞 

分 类 号：R739.86[医药卫生—肿瘤]