羊耳菊种子生活力的最佳测定条件  

Optimal Determination Method of Seed Viability of Inula cappa

作　　者：成小璐 魏怡冰 魏升华[1] 檀龙颜[1] 

机构地区：[1]贵阳中医学院药学院,贵州贵阳550002

出　　处：《贵州农业科学》2016年第8期100-104,共5页Guizhou Agricultural Sciences

基　　金：国家中医药管理局项目"国家基本药物目录原料种子种苗繁育(贵州)基地建设"(2014-2015);贵阳中医学院博士基金项目"羊耳菊野生变家种技术研究"(2015)

摘　　要：为获得羊耳菊种子生活力的最佳测定条件,采用四唑染色法,以氯化三苯基四氮唑(TTC)浓度、染色温度和染色时间为指标,通过正交试验和响应面试验对测定条件进行优化。结果表明:TTC浓度1.5%、染色温度40℃、染色时间3h为羊耳菊种子生活力测定的最佳条件,3个检测指标对种子生活力测定的影响为染色温度>染色时间>TTC浓度。In order to find out the best determination conditions of seed viability of I.cappa,the method of 2,3,5-triphenyl tetrazolium chloride（TTC） was employed with indicators of TTC concentration,dyeing temperature and dyeing time,and the determination conditions were optimized by using orthogonal method and response surface methodology.Results：1.5%,40℃,3h were the best dyeing conditions of TTC concentration,dyeing temperature and dyeing time respectively.And the order of the three factors influencing seed viability test is dyeing temperature〉dyeing time〉TTC concentration.

关 键 词：羊耳菊 种子 生活力 TTC染色法 

分 类 号：S567[农业科学—中草药栽培]