异源表达酮还原酶的重组菌单羰基还原1,2-环己二酮  

Single Carbonyl Reduction of 1,2-cyclohexanedione Catalyzed by Recombinant Strain Heterologously Expressing Ketoreductase

作　　者：李尧益 李凌凌[1] 吕早生[1] 左振宇[1] 

机构地区：[1]武汉科技大学化学与化工学院,湖北武汉430081

出　　处：《广州化工》2016年第18期62-65,104,共5页GuangZhou Chemical Industry

基　　金：湖北省科技厅基金项目(编号:2009CDA006);国家级创新创业训练计划项目(编号:201410488011)

摘　　要：利用表达糖多孢红霉菌聚酮合成酶模块1的酮还原酶的重组大肠杆菌细胞Escherichia coli BL21(p ET-ery KR1)2对1,2-环己二酮进行了催化还原反应,该还原反应为单羰基还原而非双羰基还原,生成的产物为2-羟基环己酮。对重组细胞催化1,2-环己二酮还原的反应条件进行了优化,发现最优的反应条件为底物浓度20 mmol/L,细胞密度80 g/L,p H 6.0,转速120 r/min,温度37℃,且需要添加10 g/L E.coli BL21(p ET-gdh1)辅酶再生系统和0.2 mmol/L NADPH。在该反应条件下,重组菌催化反应10 h后,底物的转化率可高达84.2%。Recombinant strain Escherichia coli BL21 （pET-eryKR1）2, which heterologously expressed ketoreductase in the first module of polyketide synthase from Saccharopolyspora erythraea, fermented with 1,2- cyclohexanedione as reduction substrate. In that reaction, the reduced product of substrate was 2-hydroxycyclohexanone, its single carbonyl was reduced. The conditions with substrate concentration of 20 mmol/L, cell density of 80 g/L, pH of 6. 0, rotation speed of 120 r/min and reaction temperature of 37℃ were optimal for this biocatalytic conversion with the addition of 10 g/L of coenzyme regeneration system E. coli BI21 （ pET-gdhl ） and 0.2 mmol/L of NADPH. The conversion rate of 1,2-cyclohexanedione catalyzed by the recombinant cells for 10 h could reach 84.2% under these conditions.

关 键 词：酮还原酶 聚酮合成酶 生物催化 1 2-环己二酮 2-羟基环己酮 

分 类 号：Q789[生物学—分子生物学] Q815