Protective Effect of Gelatin and Gelatin Hydrolysate from Salmon Skin on UV Irradiation-Induced Photoaging of Mice Skin  

作　　者：CHEN Tiejun HOU Hu LU Jiaohan ZHANG Kai LI Bafang 

机构地区：[1]College of Food Science and Engineering, Ocean University of China

出　　处：《Journal of Ocean University of China》2016年第4期711-718,共8页中国海洋大学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No.31401476);Specialized Research Fund for the Doctoral Program of Higher Education (No.20130132120024);the Fundamental Research Funds for The Central Universities (No.201313002);Shandong Province Regional Innovation and Development of Marine Economy Demonstration Projects

摘　　要：The objective of this study was to investigate the effect of gelatin(SG) isolated from salmon skin and its hydrolysate(SGH) on photoaging skin, and the mechanism responsible for anti-photoaging. The average molecular weights of SG and SGH were 65 k Da and 873 Da, respectively. The amino acid compositions of SG and SGH were similar. Both of them were abundant in hydrophobic amino acids. Twenty-five peptides were identified from SGH. SG and SGH could improve UV irradiation-induced pathological changes of macroscopical tissue texture and skin morphology. Hydroxyproline content is an indicator of matrix collagen content, SG and SGH could inhibit the decrease of hydroxyproline content in photoaging skin in a dose dependent manner. In addition, SG and SGH could alleviate UV irradiation-induced oxidative damages to skin by increasing the activities of total superoxide dismutase(T-SOD), glutathione peroxidase(GSH-Px) and catalase(CAT), increasing the content of glutathione(GSH) and decreasing the content of malonaldehyde(MDA). Moreover, SG and SGH could enhance immune regulation system by increasing the thymus index. Thus, the anti-photoaging mechanisms of SG and SGH were by inhibiting the depletion of antioxidant defense components, involving in the synthesis of collagen and enhancing the function of immune system. Besides, SGH showed a better result in protecting skin from photoaging than SG.The objective of this study was to investigate the effect of gelatin（SG） isolated from salmon skin and its hydrolysate（SGH） on photoaging skin, and the mechanism responsible for anti-photoaging. The average molecular weights of SG and SGH were 65 k Da and 873 Da, respectively. The amino acid compositions of SG and SGH were similar. Both of them were abundant in hydrophobic amino acids. Twenty-five peptides were identified from SGH. SG and SGH could improve UV irradiation-induced pathological changes of macroscopical tissue texture and skin morphology. Hydroxyproline content is an indicator of matrix collagen content, SG and SGH could inhibit the decrease of hydroxyproline content in photoaging skin in a dose dependent manner. In addition, SG and SGH could alleviate UV irradiation-induced oxidative damages to skin by increasing the activities of total superoxide dismutase（T-SOD）, glutathione peroxidase（GSH-Px） and catalase（CAT）, increasing the content of glutathione（GSH） and decreasing the content of malonaldehyde（MDA）. Moreover, SG and SGH could enhance immune regulation system by increasing the thymus index. Thus, the anti-photoaging mechanisms of SG and SGH were by inhibiting the depletion of antioxidant defense components, involving in the synthesis of collagen and enhancing the function of immune system. Besides, SGH showed a better result in protecting skin from photoaging than SG.

关 键 词：glutathione collagen superoxide hydroxyproline inhibiting irradiation thymus peroxidase antioxidant gelatin 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]