抑癌基因ST7L对卵巢癌细胞增殖、细胞周期和裸鼠移植瘤的影响  

Effects of ST7L on cell proliferation, cell cycle in ovarian cancer and tumour xenografts in nude mice

作　　者：周闻[1,2] 杨臻[1] 刘民[1] 汤华[1] 

机构地区：[1]天津医科大学基础医学院病原生物学系生命科学中心实验室,天津300070 [2]天津市天津医院检验科,天津300211

出　　处：《天津医科大学学报》2016年第5期373-376,共4页Journal of Tianjin Medical University

基　　金：国家自然科学基金资助项目(91029714;31270818;81572790);天津市自然科学基金资助项目(12JCZDJC25100)

摘　　要：目的:研究ST7L基因对人类卵巢癌细胞系OVCAR3和SKOV3细胞增殖、细胞周期和体内裸鼠成瘤模型的影响。方法:PCR扩增ST7L过表达质粒(pc DNA3/ST7L),生物合成ST7L的敲降质粒(p Silencer/ST7L);利用脂质体转染技术在卵巢癌细胞中过表达或者敲降ST7L基因,用荧光定量PCR、Western blot验证质粒的有效性;MTT、克隆形成实验、流式细胞术检测ST7L对OVCAR3和SKOV3细胞增殖能力、周期的影响;并通过裸鼠移植瘤实验研究ST7L在体内成瘤能力。结果:过表达和敲降ST7L质粒是有效的;过表达ST7L后抑制了卵巢癌细胞生长,抑制了细胞G1/S期的转化;过表达ST7L在体内能够有效抑制裸鼠的成瘤能力。结论:ST7L抑制OVCAR3和SKOV3细胞的增殖能力,抑制细胞周期进程,抑制体内裸鼠成瘤能力。Objective： To investigate the effects of ST7L on the proliferation, cell cycle in ovarian cancer and tumor xenograft in nude mice. Methods： Ectopic expression plasmid of ST7L by PCR and compounded the knockdown plasmid were established. ST7L was over- expressed and knocked down in OVCAR3 and SKOV3 cells via lipidosome 2000 transfection. Fluorescence based quantitative polymerase chain reaction and western blot assay were used to analyze the expression of ST7L. MTT, colony formation assay and flow cytometry were applied to detect the effects of ST7L on the proliferation and cell cycle of OVCAR3 and SKOV3 cells after transfection. Tumor xenograft studies were used to observe the tumor growth in vivo. Results： The used plasmids were effective; the ect0pic expression of ST7L suppressed the proliferation and cell cycle of OVCAR3 and SKOV3 cells, and the tumor growth was inhibited by over-expression of ST7L in vivo. Conclusion：ST7L could inhibit the proliferation and cell cycle of ovarian cells and tumor growth in nude mice.

关 键 词：抑癌基因ST7L 卵巢癌 移植瘤 细胞增殖 细胞周期 裸鼠 

分 类 号：R737.31[医药卫生—肿瘤]