重组hIFN-α-2b-BCG抗肿瘤效应的体外研究  

Study on antitumor effect of recombinant h IFN-α-2b-BCG in vitro

作　　者：刁呈文[1] 范晓东[1] 孙二琳[1] 李娜[1] 卢炳新[1] 雷铭德[1] 丁娜[1] 孙岩[1] 韩瑞发[1] 

机构地区：[1]天津市泌尿外科研究所,天津医科大学第二医院泌尿外科,天津300211

出　　处：《天津医科大学学报》2016年第5期377-380,385,共5页Journal of Tianjin Medical University

基　　金：国家自然科学基金资助项目(81402095);天津市科委抗癌重大科技专项基金资助项目(12zcdzsy16900);天津市卫计委科技基金资助项目(2014KZ104)

摘　　要：目的:了解重组h IFN-α-2b-BCG(r BCG)体外抗肿瘤的作用效果和作用机制。方法:体外共培养后,透射电镜观察不同时间点r BCG对人膀胱肿瘤EJ细胞的影响。吖啶橙染色观察各组肿瘤细胞形态。MTT法检测r BCG对肿瘤细胞生长抑制率。ELISA检测r BCG作用后淋巴细胞分泌Th1型细胞因子水平。LDH释放试验检测r BCG激活的淋巴细胞对肿瘤细胞杀伤效应。结果:BCG和r BCG作用后的肿瘤细胞在透射电镜下和吖啶橙染色后均有显著变化。MTT显示r BCG的生长抑制率显著高于BCG和BCG+IFN-α-2b组。r BCG对淋巴细胞分泌Th1型细胞因子有影响,同时r BCG激活的淋巴细胞对膀胱肿瘤细胞有杀伤作用。结论:重组BCG在体外有优于BCG的免疫调节特性、抗肿瘤作用和直接细胞毒作用。Objective： To study antitumor effect ahd mechanism of action on recombinant IFN-α-2b-BCG （rBCG）in vitro. Methods： Effect of rBCG on bladder tumor cells was observed through transmission electro microscopy and microscope. Acridine orange staining was used to observe the tumor cellsmorphology by fluorescence microscope. Growth inhibition rate on tumor cell was detected by MTT assay. Effect of rBCG cultures on secretion of Thl -type cytokines was detected by ELISA assay. LDH release assay was applied to determine the lethal effect of active lymphocyte which was stimulated by BCG on tumor cells. Results： The rBCG group showed significant changes. MTT showed rBCG inhibited the proliferation of bladder cancer cell, and more active other groups. RBCG cultures affected the section ofThl cytokines. And rBCG could enhanced the lethal effect of lymphocyte on tumor cells. Conclusion： The rBCG may have more immunomodulatory properties, anti-tumor effects and cytotoxicity in vitro.

关 键 词：重组BCG IFN-Α-2B 膀胱肿瘤 免疫疗法 

分 类 号：R737.14[医药卫生—肿瘤]