猪殃殃抗苯磺隆分子机制研究  

Molecular Mechanism of Galium aparine Resistance to Tribenuron-Methyl

作　　者：张乐乐[1] 李琦[1] 郭文磊[1] 李伟[1] 王金信[1] 

机构地区：[1]山东农业大学植物保护学院,山东泰安271018

出　　处：《中国农学通报》2016年第25期110-113,共4页Chinese Agricultural Science Bulletin

基　　金：国家公益性行业(农业)科研专项"杂草抗药性监测及治理技术研究与示范"(201303031);国家自然科学基金面上项目"基于菵草ACCase等位基因突变的适合度代价研究"(31471787)

摘　　要：近几年,河南省各地抗苯磺隆猪殃殃(Galium aparine)发生严重,常规剂量苯磺隆已无法防治。为了明确猪殃殃产生苯磺隆抗性的分子机制,通过提取总DNA、ALS基因扩增和基因测序的方法比对抗性及敏感种群的ALS保守区序列,明确抗性种群ALS基因突变位点。相比于敏感猪殃殃,抗性种群的高度保守区(Domain A)197位脯氨酸(Pro)突变为苏氨酸(Thr)和亮氨酸(Leu),非保守区457苏氨酸(Thr)突变为丝氨酸(Ser)3种突变形式。其中,猪殃殃对苯磺隆产生抗药性的原因可能是其197位氨基酸发生突变。In recent years, the tribenuron-methylin resistant C, alium aparine was serious throughout Henan Province, and common dose of tribenuron-methyl cannot control Galium aparine any more. The objective of this study is to understand the molecular mechanism of the Galium aparine resistance to tribenuron-methyl. Through total DNA extraction, ALS clone and sequencing, the authors found the specific mutation sites in amino acid sequence of acetolactate synthase （ALS） in the resistant biotype of Galium aparine. Compared with the susceptible biotype, there were three types of amino acid substitutions in the resistant biotypes, of which, the amino acid substitution of Pro197 （CCC） to Thr （ACC） and Pro197 （CCC） to Leu （CTC） were located in the highly conserved region Domain A, Thr 457 （ACC） to Ser （TCC） in the non-conservative region. The substitution of Pro197 might be the reason for the resistance in the R-biotype of Galium aparine to tribenuron- methyl.

关 键 词：猪殃殃 乙酰乳酸合成酶抑制剂 抗药性 基因突变 

分 类 号：S481.4[农业科学—农药学]