白介素17基因启动子区CpG岛甲基化与宫颈癌的相关性研究  

Correlation between CpG Island Methylation in IL-17 Gene Promoter and Cervical Cancer

作　　者：袁陆 张丽杰[2] 李鸥[2] 杨艳艳[2] 张琪 王新月 耿美丽[2] 姜春阳[3] 王洋 马冬 

机构地区：[1]华北理工大学河北省煤炭职业卫生与安全重点实验室,河北省唐山市063000 [2]唐山工人医院妇科 [3]天津市人民医院胸外科

出　　处：《中国全科医学》2016年第27期3300-3305,共6页Chinese General Practice

基　　金：国家自然科学基金资助项目(81541149);河北省青年科学自然基金(H 2016209046)

摘　　要：目的探讨白介素17（IL-17）基因启动子区Cp G岛甲基化与宫颈癌的相关性,为临床诊断和免疫治疗宫颈癌提供新思路。方法选取2011年3月—2012年5月在唐山工人医院住院部就诊的宫颈癌患者43例（宫颈癌组）、癌前病变患者62例〔其中宫颈上皮内瘤变（CIN）Ⅰ级23例（CINⅠ组）、CINⅡ~Ⅲ级39例（CINⅡ~Ⅲ组）〕,另选取本院同期因子宫肌瘤行全子宫切除术的患者43例作为对照组。4组患者宫颈组织标本均经手术获得。提取4组宫颈组织DNA并进行高危型人乳头瘤病毒（HPV）16 DNA检测,采用Cp G岛甲基化特异性PCR（MSP）法检测IL-17基因启动子区Cp G岛甲基化状态,采用半定量反转录PCR（RT-PCR）法检测IL-17 mRNA表达水平,分析IL-17基因启动子区Cp G岛甲基化率与IL-17 mRNA表达水平的相关性及其与HPV16 DNA阳性率的关联性,并分析不同年龄、临床特征宫颈癌患者IL-17基因启动子区Cp G岛甲基化率。结果 4组HPV16 DNA阳性率比较,差异有统计学意义（P〈0.001）。CINⅡ~Ⅲ组、宫颈癌组IL-17基因启动子区Cp G岛甲基化率低于对照组（P〈0.01）;CINⅠ组与对照组、CINⅡ~Ⅲ组、宫颈癌组IL-17基因启动子区Cp G岛甲基化率比较,差异无统计学意义（P〉0.05）;CINⅡ~Ⅲ组与宫颈癌组IL-17基因启动子区Cp G岛甲基化率比较,差异无统计学意义（P〉0.05）。CINⅠ组、CINⅡ~Ⅲ组和宫颈癌组IL-17 mRNA表达水平均高于对照组（P〈0.05）;CINⅠ组与CINⅡ~Ⅲ组、宫颈癌组IL-17 mRNA表达水平比较,差异无统计学意义（P〉0.05）;CINⅡ~Ⅲ组与宫颈癌组IL-17 mRNA表达水平比较,差异无统计学意义（P〉0.05）。根据IL-17基因启动子区Cp G岛甲基化检测结果将所有患者分为IL-17基因启动子区Cp G岛甲基化患者（93例）和非甲基化患者（55例）,IL-17基因启动子区Cp G岛非甲基化患者IL-17 mRNA表达水平高于甲基化患者（P〈0.05）。所有患者宫颈组织�Objective To investigate the correlation between Cp G island methylation of IL-17 gene promoter and cervical cancer,and to provide new ideas for clinical diagnosis and immunotherapy of cervical cancer. Methods 43 cervical cancer patients（ cervical cancer group） and 62 patients with precancerous lesions 〔23 cases with CIN Ⅰ（ CINⅠ group）,39 cases with CINⅡ～Ⅲ（ CINⅡ～Ⅲ group） 〕who received treatment in Inpatient Department of Tangshan Gongren Hospital from March 2011 to May 2012 were selected,and another 43 patients who had undergone total abdominal hysterectomy in this hospital at the same period due to uterine fibroids were enrolled as control group. Cervical tissue specimens of patients in four groups were obtained by surgery. DNA in cervical tissue of patients in four group was extracted to make a detection of HPV16 DNA,Cp G island methylation status in IL-17 gene promoter was detected by using Cp G island methylation specific PCR（ MSP）, IL-17 mRNA expression levels were detected by semi- quantitative reverse transcription PCR（ RT- PCR）,correlation between Cp G island methylation rate of IL-17 gene promoter and IL-17 mRNA expression levels,and the relevance of this rate to the positive rate of HPV16 DNA were analyzed, and Cp G island methylation status of IL-17 gene promoter in cervical cancer patients of different ages and clinical features were also analyzed. Results HPV16 DNA positive rates in four groups were significantly different（ P〈0. 001）. Cp G island methylation rate of IL-17 gene promoter in CINⅡ～Ⅲ group and cervical cancer group was significantly lower than that in control group（ P〈0. 01）; Cp G island methylation rates of IL-17 gene promoter in CINⅠ group and control group,CINⅠ group,CINⅡ～Ⅲ group,cervical cancer group were not significantly different（ P〈0. 05）; Cp G island methylation rates of IL-17 gene promoter between CINⅠgroup and CINⅡ～Ⅲ group were not significantly different（ P〈0. 05）. The expression levels of

关 键 词：宫颈肿瘤 白细胞介素17 DNA甲基化 CP G岛 人乳头瘤病毒16 

分 类 号：R737.33[医药卫生—肿瘤]