胶质瘤中MicroRNA-374的表达及其临床意义  

Expression and clinical significance of miR-374 in glioma tissues

作　　者：沈云龙[1] 安泰学 李和珍[1] 杨勇[1] 王雪娟[1] 刘丽红[1] 

机构地区：[1]南方医科大学第五附属医院,广东广州510900 [2]南方医科大学

出　　处：《解剖学研究》2016年第4期289-293,共5页Anatomy Research

基　　金：广东省科技计划项目(2013B021800050);广东省科技计划项目(2014A020212727);广州市科技和信息化局应用基础研究项目(2013J4100013)

摘　　要：目的研究人脑胶质瘤组织中微小RNA-374(microRNA-374,miR-374)的表达及其与临床病理特征之间的关系,探讨其在胶质瘤患者中的临床意义。方法采用RT-PCR法检测85例胶质瘤和30例正常脑组织中的miR-374表达;Kaplan-Meier法分析胶质瘤患者的总生存期;观察慢病毒过表达miR-374载体转染U251和U87细胞株后对肿瘤细胞增殖的影响。结果胶质瘤组织中miR-374的表达明显下调;miR-374的表达与WHO分级(P=0.008)、Karnofsky评分(KPS)(P=0.021)和生存时间(P=0.01)显著相关;Kaplan-Meier分析结果表明,miR-374低表达与高表达患者总生存期(P<0.01)的差异有统计学意义,miR-374低表达的患者预后较差。多因素分析显示胶质瘤中miR-374的差异表达是预测胶质瘤患者预后的独立危险因素(HR:5.75,95%CI:3.56-6.93,P=0.005)。体外实验表明,过表达miR-374可以显著抑制胶质瘤细胞的增殖。结论 miR-374在胶质瘤中是低表达的,与胶质瘤患者预后显著相关;miR-374高表达可抑制胶质瘤细胞的增殖。Objective To investigate the relationship between microRNA-374 （miR-374） expression and clinicopatholog- ic factors in glioma tissues, and to evaluate the clinical significance of miR-374 in glioma patients. Methods The expression lev- els of miR-374 were analyzed in 85 glioma tissues and 30 normal brain tissues by real-time PCR （RT-PCR）. Survival analysis by Kaplan-Meier method was performed to assess prognostic significance. And miR-374 over-expression lentiviral vector （Lv-miR- 374） and a control lentiviral empty vector （LEV） were stably transfected into human glioma U251 and U87 cells, to evaluate the ef- fect of niR-374 on cell proliferation. Results Down-regulation of miR-374 was detected in most primary glioma tissues, and which was significantly associated with WHO grade（P=0.008）, Karnofsky performance scorc（KPS）（P=0.021 ）, and survival time（P=0.01 ）.Using Kaplan-Meier analysis, we found that low expression of miR-374 was associated with a worse prognosis. Multivariate analyses showed that miR-374 expression was the independent risk factors for predicting overall survival time of glioma patients. Conclusion MiR-374 is down-regulated in glioma tissues, miR-374 expression is significantly correlated with the prognosis of glio- ma patients, and up-regnlation miR-374 in U251 and U87 cells could inhibit the prolifrration of glioma cells.

关 键 词：胶质瘤 miR-374 U251细胞株 U87细胞株 增殖 

分 类 号：R739.41[医药卫生—肿瘤]