HuR和HIF-1α在肝癌组织中的表达及其与患者的预后的关系  

Expressions of HuR and HIF-1α in hepatocellular carcinoma tissues and their association with prognosis of patients

作　　者：雷达[1] 李永利[1] 祁军安[1] 

机构地区：[1]宝鸡市中心医院肝胆胰脾外科,陕西宝鸡721008

出　　处：《中国临床研究》2016年第9期1160-1163,共4页Chinese Journal of Clinical Research

基　　金：陕西省科学技术研究计划(2011k12-67)

摘　　要：目的探讨RNA结合蛋白人抗原R(HuR)和转录因子缺氧诱导因子-1α(HIF-1α)在肝癌组织中的表达及其与患者预后的关系。方法收集55例行肝癌手术切除后保存完好的肝癌组织和癌旁组织蜡块,同时选取20例正常人的肝脏组织作为正常对照,采用免疫组化染色法检测不同组织中HuR和HIF-1α蛋白表达阳性率,采用Spearman相关分析研究肝癌组织中HuR和HIF-1α蛋白表达的相关关系,采用Kaplan-Meier法生存分析肝癌组织HuR和HIF-1α蛋白表达与患者预后的关系。结果肝癌组织、癌旁组织和正常肝组织中HuR蛋白的阳性表达率分别为67.27%、20.0%和5.0%,3种组织中HuR蛋白阳性表达率的差异具有统计学意义(P<0.01);肝癌组织、癌旁组织和正常肝组织中HIF-1α蛋白的阳性表达率分别为58.18%、12.73%和10.0%,肝癌组织中HIF-1α蛋白阳性表达率高于癌旁组织和正常肝组织,差异有统计学意义(P<0.01)。经Spearman相关分析,肝癌组织HuR蛋白表达与HIF-1α蛋白表达呈正相关(rs=0.508,P<0.01);Log-rank检验表明,HuR蛋白表达阳性的肝癌患者的5年随访中位无瘤生存期为27个月,显著低于HuR蛋白表达阴性患者的32个月(log-rank=19.350,P<0.01);HIF-1α蛋白表达阳性的肝癌患者的5年随访中位无瘤生存期为28个月,显著低于HIF-1α蛋白表达阴性患者的37个月(log-rank=26.556,P<0.01)。结论肝癌组织中HuR和HIF-1α表达率异常增高,两者在肝癌组织中的表达呈正相关,且与患者的无瘤生存时间密切相关。Objective To observe the expressions of human antigen R（ HuR, a member of RNA binding protein） and hy- poxia inducible factor-1 α（ HIF-1α, a treanscription factor） in hepatocellular carcinoma （HCC）tissues and their association with the prognosis of patients. Methods The well-preserved wax block samples of HCC tissues and para-carcinoma tissues after surgical resection from 55 cases of HCC were collected, and the liver tissues samples from 20 normal subjects were selected as controls. Immunohistochemical staining method was used to detect the positive expression rates of HuR and HIF-1α proteins in different tissues. Spearman correlation analysis was used to analyze the relationship between the HuR and HIF-1α proteins expressions in HCC tissues. Kalplan-Meier survival analysis was used to analyze the correlation of expressions of HuR and HIF-1α proteins in HCC tissues with the patients＇ prognosis. Results There were significant differences in positive expression rates of HuR protein in carcinoma tissues, para-carcinoma tissues and normal liver tissues（67.27% , 20.00% ,5.00% ;P 〈 0. 01 ）. There were significant differences in positive expression rates of HIF-1α protein in carcinoma tissues,para-carcinoma tissues and normal liver tissues（58.18% , 12.73% , 10.00% ;P 〈 0.01 ）. Spearman correlation analysis showed that the expression of HIF-1 protein in HCC tissues was positively correlated with the expression of HuR protein in HCC tissues （rs = 0. 508 ,P 〈 0.01 ）. Log-rank test showed that the median tumor-free survival time in follow-up of 5 years in HCC patients with positive HuR protein expression was significant lower than that in patients with negative HuR protein expression（ 27 months vs 32 months, Log-rank = 19. 350, P 〈 0.01 ） ; the median tumor-free survival time in follow-up of 5 years in HCC patients with positive HIF-1 c~ protein expression was significant lower than that in patients with negative HIF-lct protein expression（28 months vs 37 months,log-rank = 26.

关 键 词：原发性肝癌 人抗原R 缺氧诱导因子-1Α 无瘤生存期 预后 相关性 

分 类 号：R735.7[医药卫生—肿瘤]