抑癌基因p53、凋亡抑制基因Bcl-2、促凋亡基因Bax在胃癌及癌前病变中的表达  

Expression of tumor suppressor gene p53,apoptosis-suppressing gene Bcl-2,proapoptotic gene Bax in gastric cancer and precancerous lesions

作　　者：朱玉侠[1] 赵明星[1] 姜登鸽 时永全 

机构地区：[1]西安市第一医院消化科,陕西西安710002 [2]西京消化病医院消化科

出　　处：《胃肠病学和肝病学杂志》2016年第9期1040-1043,共4页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的研究抑癌基因p53、凋亡抑制基因Bcl-2、促凋亡基因Bax在胃癌及癌前病变中的表达,探讨三种基因在胃癌发生、发展中的作用。方法采用免疫组化(IHC)方法对90例慢性萎缩性胃炎患者、79例并发肠上皮化生患者、90例异型增生患者、113例胃癌患者的p53、Bcl-2和Bax的表达情况进行分析。结果胃癌组p53阳性表达率显著高于其他三组,差异有统计学意义(P<0.01);胃癌组Bcl-2阳性表达率显著高于慢性萎缩性胃炎组和伴肠上皮化生组(P<0.01);胃癌组Bax阳性表达率低于慢性萎缩性胃炎组,差异有统计学意义(P<0.05)。p53、Bcl-2、Bax基因蛋白表达在患者的性别、年龄、组织学类型、肿瘤体积、淋巴结转移、发病部位及临床分期等方面差异均无相关性(P>0.05)。结论 p53的过度表达是胃癌发生过程较早期事件,Bcl-2的表达逐渐增加,而Bax的表达逐渐下降,在胃癌发生过程中可能存在凋亡抑制。Objective To investigate the expression of tumor suppressor gene p53, apoptosis-suppressing gene Bcl- 2, pro-apoptotic gene Bax in gastric cancer and precancerous lesions, and explore the role of 3 genes in the development of gastric cancer. Methods Expressions of p53, Bcl-2 and Bax in 90 cases of chronic atrophic gastritis, 79 cases of intestinal metaplasia, 90 cases of dysplasia and 113 cases of gastric cancer were detected by immunohistochemistry （IHC）. Results The positive expression rate of p53 in gastric cancer group was significantly higher than that in the other three groups, the difference was statistically significant （P 〈 0.01 ）. The positive expression rate of Bcl-2 in gastric cancer group was significantly higher than that in chronic atrophic gastritis group and intestinal metaplasia group （ P 〈 0.01 ）. The positive expression rate of Bax in gastric cancer group was lower than that in chronic atrophic gastritis group, the difference was statistically significant （P 〈 0.05）. There were no correlations between p53, Bcl-2, Bax gene expression and gender, age, histological type, tumor size, lymph node metastasis, incidence and clinical stage and so on （P 〉 0.05）. Conclusions Overexpression of p53 is an earlier event in gastric cancer, the expression of Bel-2 increased gradually, while the expression of Bax decreased gradually. In the process of gastric cancer, there may be an inibition of apoptosis.

关 键 词：p53 BCL-2 BAX 基因表达 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]