量子点荧光标志结肠癌细胞及在其组织芯片的应用价值  

Application of quantum dots immune fluorescent labelling in colorectal cancer tissues

作　　者：何凤屏[1] 徐新[1] 吴青松[1] 李定云[1] 马占忠[1] 郭艳乐[1] 唐盛[2] 尹卫东 龚海涛 刘艺 林恒先 

机构地区：[1]汕头大学医学院附属粤北人民医院,广东汕头512026 [2]广东省深圳市迈科龙生物技术有限公司,518057

出　　处：《国际检验医学杂志》2016年第18期2531-2533,共3页International Journal of Laboratory Medicine

基　　金：广东省重大科技专项基金资助项目(2013A022100011)

摘　　要：目的探讨新的分子荧光标志量子点(QDs)在结肠癌组织芯片上进行不同蛋白的检测及评价其临床作用。方法在结肠癌组织芯片上,利用QDs标志的链霉亲和素复合物(QDs-SA)能与生物素化二抗IgG荧光结合特点进行免疫荧光组织化学检测K-ras、MXRA5蛋白表达,评价其在蛋白定位情况。结果观察到K-ras、MXRA5蛋白在结直肠癌组织呈高表达,并定位于结肠癌细胞的细胞膜和胞核。结论 QDs具有很好的荧光作用,能与抗体紧密结合。采用链霉亲和素修饰的QDs,能够准确地检测结肠癌组织芯片上不同蛋白的定位,可为结肠癌体内和体外临床诊断提供新方法。Objective To investigate different antigens detected by a novel labelled reagent-quantum dots（QDs）in the colorectal cancer tissues microarray（TMA）.Methods Depend on QDs streptavidin conjugate（QDs-SA）combined specially with biotinylation IgG,immune of luorescent histochemistry was utilized to examine expression of K-ras,matrix-remodeling associated 5（MXRA5）proteins in the colorectal cancer TMA,where the protein accurate location was observed.Results K-ras,matrix-remodeling associated 5（MXRA5）proteins were high expressed in colorectal cancer tissue and located accurately in the cell membrane and nucleus of colorectal cancer cells,respectively.Conclusion QDs exhibit excellent photostability,broad emission spectrum and long fluorescence lifetime.Modified with streptavidin could accurately detect different protein locations in the colorectal cancer TMA.This is a novel approach for studying targeted imaging of colorectal cancer in vivo and vitro clinical diagnosis.

关 键 词：量子点荧光标志 结肠癌 K-ras蛋白 MXRA5蛋白 

分 类 号：R735.35[医药卫生—肿瘤]