口腔与胃内幽门螺杆菌感染的相关性研究  

The correlation between helicobacter pylori infection in oral cavity and stomach

作　　者：万洋洋[1] 陈相[1] 赵枰[1] 王朔[1] 

机构地区：[1]江苏省南通市第一人民医院,226001

出　　处：《国际检验医学杂志》2016年第18期2566-2567,2570,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨口腔与胃幽门螺杆菌感染的相关性。方法用幽门螺杆菌的特异性引物及特异Taqman探针进行荧光定量聚合酶链反应(FQ-PCR)检测27例根除治疗失败患者牙菌斑和胃黏膜幽门螺杆菌DNA,并进一步运用单链构象多态性(SSCP)分析技术分析口腔和胃内幽门螺杆菌菌株DNA基因型。结果 FQ-PCR:27例胃黏膜及牙菌斑标本采用FQ-PCR检测幽门螺杆菌,胃黏膜均为阳性;牙菌斑25例阳性,2例阴性。SSCP:胃黏膜和牙菌斑幽门螺杆菌基因型相同者(SSCP带型相同)19例,其余6例两者至少有1种相同的SSCP带型。结论同一个体牙菌斑和胃黏膜中的幽门螺杆菌属同种菌株的可能性大。口腔幽门螺杆菌可能是胃幽门螺杆菌感染的重要来源。胃幽门螺杆菌感染与口腔幽门螺杆菌感染密切相关。Objective To explore relationship between helicobacter pylori（Hp）infection in oral cavity and stomach.Methods A fluorogenic quantitative polymerase chain reaction（FQ-PCR）assay with specific primers and specific Taqman probe was used to detect Hp in dental plaque and gastric biopsy in 27 patients with eradication failure.Then the products were futher processed by single-strand conformation polymorphism（SSCP）.Results（1）FQ-PCR：we detected Hp in 27 gastric biopsy samples and dental plaque using fluorogenic quantitative polymerase chain reaction（FQ-PCR）technique.As the results,positive results could be found in 27 gastric biopsy samples.25 of positive results and 2of negative results could be found in 27 dental plaque samples.（2）SSCP：Hp can be detected both in gastric biopsy samples and denta plaque in 25 patients,and 19 of them have the same genotype of Hp,since the banding paterns detected by SSCP method were all same in Hp from gastric biopsy and denta plaque.At least one specific banding patern by SSCP was found to be same in the Hp from dental plaques and gastric biopsy of other 6patients.ConclusionThe results suggest that the genotype in gastric biopsy samples and dental plaque in one person may be same.Oral Hp infection may be an important source of gastric Hp infection.The Hp infection in stomach might be closely relevant to the infection in oral cavity.

关 键 词：幽门螺杆菌 荧光定量聚合酶链反应 多态性分析 

分 类 号：R573[医药卫生—消化系统]