电针对缺血再灌注大鼠脑内神经干细胞分化的影响  

Effects of Electroacupuncture on Differentiation of Neural Stem Cells after Cerebral Ischemia-reperfusion in Rats

作　　者：王露露[1,2,3] 林云娇 吴洁[1,2,3] 叶晓倩[1,2,3] 黄佳[1,2,3] 柳维林[1,2,3] 陶静[1,2,3] 陈立典[1,2,3,4] 

机构地区：[1]福建中医药大学康复医学院,福建福州市350122 [2]福建康复产业研究院技术创新平台,福建福州市350122 [3]福建省康复技术重点实验室,福建福州市350122 [4]国家中医药管理局中医康复研究中心,福建福州市350122

出　　处：《中国康复理论与实践》2016年第9期993-998,共6页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金项目(No.81373778;No.81273835);福建省卫生厅青年科研课题(No.2014-1-70)

摘　　要：目的观察电针对缺血再灌注大鼠神经干细胞分化的影响,探讨电针发挥脑保护作用的可能机制。方法 36只雄性Sprague-Dawley大鼠随机分为假手术组(n=12)、模型组(n=12)和电针组(n=12)。模型组与电针组均按照改良Longa法制备左侧大脑中动脉阻断90 min后再灌注模型。电针组行电针曲池、足三里干预21 d。电针后7 d、14 d、21 d行改良神经功能评分。电针后21d小动物磁共振扫描仪T2WI观察大鼠脑梗死体积;Brd U/Dcx、Brd U/Neu N免疫荧光观察缺血侧神经干细胞分化情况。结果电针后7 d、14 d、21 d,电针组神经功能评分差值高于同时间点模型组(P<0.05)。电针后21 d,电针组脑梗死体积小于模型组(P<0.05);室管膜下区Brd U^+/Dcx^+细胞数显著少于模型组(P<0.001),缺血周围皮质区Brd U^+/Neu N^+细胞数显著多于模型组(P<0.001)。结论电针曲池、足三里可以改善缺血再灌注大鼠神经功能缺损评分,减小脑梗死体积;可能与促进增殖的细胞向神经元分化有关。Objective To explore the effect of electroacupuncture at Quchi （LI11）, Zusanli （ST36） on differentiation of neural stem cells after cerebral ischemia-reperfusion in rats. Methods Thirty-six male Sprague-Dawley rats were randomly divided into sham group （n=12）, model group （n=12） and electroacupuncture group （n=12）. The latter two groups were occluded the left middle cerebral arteries for 90 min-utes and reperfused. The electroacupuncture group received electroacupuncture at Quchi and Zusanli acupoints for 21 days. They were evalu-ated with modified Neurological Severity Scores 7, 14 and 21 days after electroacupuncture. Their infarct volumes were tested with MRI T2WI 21 days after electroacupuncture, while the differentiation of neural stem cells was observed with double-immunopositive BrdU/Dcx and BrdU/NeuN. Results Compared with the model group, the neurological deficits score improved in the electroacupuncture group in all the time points （P〈0.05）. The infarct volumes decreased in the electroacupuncture group （P〈0.05）, with less number of BrdU＋/Dcx＋cells in subventricular zone （P〈0.001） and more number of BrdU＋/NeuN＋ cells in peri-infarct cortex （P〈0.001） 21 days after electroacupuncture. Conclusion Electroacupuncture at Quchi and Zusanli acupoints can improve neurological function and decrease the infarct volumes in rats after cerebral ischemia-reperfusion, which may be associated with promoting differentiation of neural stem cells to neurons.

关 键 词：脑缺血再灌注 电针 神经干细胞 分化 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]