LncRNA KCNQ1OT1在非小细胞肺癌组织中的表达及其临床意义  

Expression of Long Non-Coding RNA KCNQ1OT1 and Its Clinical Significance in Non-Small Cell Lung Cancer Tissues

作　　者：李静[1] 刘晓琴[2] 杨业[2] 柳斌[2] 

机构地区：[1]四川省肿瘤医院第二门诊部,四川成都610041 [2]四川省肿瘤医院肺部肿瘤病区,四川成都610041

出　　处：《中国呼吸与危重监护杂志》2016年第5期453-457,共5页Chinese Journal of Respiratory and Critical Care Medicine

摘　　要：目的探讨长链非编码RNA(LncRNA)KCNQ1OT1在非小细胞肺癌(NSCLC)肺组织中的表达及临床意义。方法收集2011年1月至2013年12月期间在四川省肿瘤医院行手术治疗的89例NSCLC患者的临床资料,采用实时荧光定量PCR法检测肺癌组织以及距离癌组织5 cm以上癌旁组织中LncRNA KCNQ1OT1的表达,结合临床资料分析其临床意义。结果与癌旁组织比较,LncRNA KCNQ1OT1在NSCLC组织中的表达水平明显增高(P<0.001)。单因素分析显示LncRNA KCNQ1OT1的表达与患者年龄、性别及病理类型无关(P>0.05),与肿瘤大小(X^2=12.619,P<0.001)、淋巴结转移(X^2=10.298,P=0.001)、TNM分期(X^2=7.199,P=0.007)及吸烟史(X^2=24.005,P<0.001)明显相关。生存分析提示LncRNA KCNQ1OT1高表达患者的总生存时间(20.0个月比35.0个月,X^2=45.860,P<0.001)和中位无进展生存时间(12.0个月比24.0个月,X^2=31.510,P<0.001)均显著低于低表达患者。Cox回归分析发现疾病分期和LncRNA KCNQ1OT1的表达可作为独立的预后不良的标志。结论 LncRNA KCNQ1OT1在NSCLC组织标本中高表达,与患者的预后相关,可作为潜在的肺癌预后预测分子标志物。Objective To explore the expression of long non-coding RNA （LncRNA） KCNQ10T1 and its clinical significance in non-small cell lung cancer （NSCLC）. Methods Eighty-nine NSCLC patients who underwent surgery were recruited in Sichuan Cancer Hospital from January 2011 to December 2013. Quantitative real-time PCR was used to detect the expression of LncRNA KCNQ1OT1in tumor tissues and paracarcinoma tissues （5cm or above away from tumor）. The relationship between LncRNA KCNQ1O1 expression and clinicopathologic features was analyzed by univariate analysis and Cox regression analysis. Results The expression of LncRNA KCNQ1OT1 significantly increased in tumor tissues than that in paracarcinoma tissues （ P 〈 0. 001 ）. The patients were divided into a high expression group and a low expression group according to the relative expression of LncRNA KCNQ1OT1. Univariate analysis showed that the differences between two groups were not significant in age, gender or histological type, but were significant in tumor size （χ2 = 12. 619, P 〈 0. 001 ）, lymph node metastasis （χ2 = 10. 298, P = 0. 001 ）, TNM stage （ χ2 = 7. 199, P = 0. 007 ）, and history of smoking （ χ2= 24. 005, P 〈 0. 001 ）. Kaplan-Meier analysis showed the patients with high LncRNA KCNQ1OT1 expression had significantly lower overall survival time （20. 0 months vs. 35.0 months,χ2 =45. 860,P 〈 0. 001 ） and significantly lower progression-free survival time （12. 0 months vs. 24. 0 months, Z2 = 31. 510, P 〈 0. 001 ） than those with low LncRNA KCNQ1OT1 expression. Cox regression analysis revealed that the disease stage and the expression of LncRNA KCNQ1OT1 could be used as independent prognostic markers for poor prognosis. Conclusion LncRNAKCNQ1OT1 is highly expressed in tumor tissues and associated with the prognosis of NSCLC patients, thus can be used as a potential marker for predicting the prognosis of lung cancer.

关 键 词：LncRNA KCNQ1OT1 非小细胞肺癌 预后 

分 类 号：R734.2[医药卫生—肿瘤]