银杏叶提取物诱导骨髓间充质干细胞成骨分化过程中P38信号通路的研究  

Ginkgo Biloba Extract Promotes Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells in a Pathway Involving P38 MAPK Signaling Pathway

作　　者：时舒曼 袁浩天 张晓晓[1] 吴哲[1,3] 

机构地区：[1]吉林大学口腔医学院修复科吉林长春130021 [2]中国人民解放军208医院吉林长春130000 [3]广州医科大学附属口腔医院广东广州510500

出　　处：《口腔医学研究》2016年第9期912-915,共4页Journal of Oral Science Research

基　　金：吉林省产业技术研究与开发项目(编号:2013C023-5)

摘　　要：目的:探讨银杏叶提取物(ginkgo biloba extract,GBE)促进大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)成骨分化的机制。方法:将150mg/L GBE作用于第3代大鼠BMSCs,抑制组使用P38丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路抑制剂SB203580预处理,通过茜素红(alizarin red S,ARS)染色、碱性磷酸酶(Alkalinet phosphatase,ALP)法以及RT-PCR等检测方法判定GBE是否通过上调P38信号通路促进大鼠BMSCs成骨分化。结果:与实验对照组大鼠相比,抑制组的大鼠BMSCs细胞外基质矿化减少,ALP表达明显下降,成骨相关基因ALP、BMP-2、OCN、Runx2(runt-related transcription factor2)的表达量亦明显降低。结论:GBE通过上调P38信号通路促进大鼠BMSCs成骨分化。Objective：To reveal the mechanism of GBE promoting osteogenic differentiation of rat BMSCs.Methods：150mg/L GBE was applied to the third generation of rat BMSCs,while the inhibitor group underwent a pretreatment of SB203580,which was the specific inhibitor of P38 MAPK signaling pathway.The results were evaluated by ARS dye,ALP activity assay and RT-PCR.Results：Statistically significant difference was found between the control group and the inhibitor group.In the inhibitor group,extracellular calcium deposition,ALP activity and the expression of osteogenic-related genes including ALP,BMP-2,OCN and Runx2 were lower than the control group.Conclusion：GBE promoted osteogenic differentiation of rat BMSCs by upregulating the P38 MAPK signaling pathway.

关 键 词：银杏叶提取物 骨髓间充质干细胞 P38MAPK 信号通路 成骨分化 

分 类 号：R580[医药卫生—内分泌]