CYP2W1在胃癌组织中的表达及对胃癌细胞增殖和侵袭的影响  

Expression of CYP2W1 and its effect on proliferation and invasion of gastric cancer

作　　者：黄天臣[1] 肖建安[1] 王青兵[1] 王雁军[1] 张勇[1] 白东晓[1] 冯留顺[2] 

机构地区：[1]安阳市肿瘤医院外四科,河南省455000 [2]郑州大学第一附属医院普通外科

出　　处：《中华普通外科杂志》2016年第9期771-773,共3页Chinese Journal of General Surgery

摘　　要：目的探讨CYP2W1在胃癌组织中的表达情况，研究其对胃癌细胞增殖和侵袭的影响。方法采用免疫组织化学技术检测326例胃癌组织及相应正常胃黏膜组织中CYP2W1蛋白的表达：采用半定量RT—PCR实验检测随机选取10例胃癌组织和相对应的正常胃黏膜组织中CYP2W1mRNA的表达：构建4组胃癌及正常胃黏膜细胞系，应用Westernblot方法检测各组细胞系中CYP2W1蛋白的表达；应用MTT实验和Transwell侵袭实验检测其对胃癌细胞增殖和侵袭能力的影响。结果胃癌组织中CYP2W1蛋白阳性表达率高于正常胃黏膜组织（26．7％比0，X^2=100．396，P〈0．05）。胃癌组织中CYP2w1mRNA高于正常胃黏膜组织[（0．413±0．026）比（0．074±0．005），t=28．115，P〈0．05]。胃癌细胞中CYP2W1蛋白的表达高于正常胃黏膜细胞[（0．481±0．024）比0，t=49．097，P〈0．05]。CYP2W1阳性胃癌细胞生长能力高于CYP2W1阴性胃癌细胞（P〈0．05）。24hCYP2W1阳性胃癌细胞侵袭穿过Transwell小室基质胶的细胞数量高于CYP2W1阴性胃癌细胞[（63±8）比（18±3），t=24．134，P〈0．05]。结论CYP2WI仅在胃癌组织中表达，在正常胃组织中不表达；CYP2W1与胃癌细胞的增殖和侵袭能力相关。Objective To investigate the expression of CYP2W1 in gastric cancer and its effect on proliferation and invasion. Methods CYP2W1 protein expression in 326 gastric cancertissues and in the corresponding normal gastric mucosa was measured by immunohistochemstry. The expression of CYP2W1 mRNA in 10 randomly chosen gastric cancer tissues and its corresponding normal gastric mucosa was tested by semi-quantitative RT-PCR. 4 groups pairs of gastric cancer and normal gastric mucosa cell lines were constructed. CYP2W1 expression in each of the cell line was tested. The effect of CYP2W1 expression on the proliferation and invasion capacity of the gastric cancer cells was studied by MTT experiment and transwell cell experiment. Results Expression of CYP2W1 protein in the gastric cancer tissues is higher than that in normal gastric mucosa （26.7% vs. 0, X^2 = I00. 396, P 〈 0. 05 ）. CYP2WI mRNA in the gastric cancer tissues is higher than that in normal gastric mucosa [ （0. 413 ＋ 0. 026） vs. （0. 074 ±0. 005 ）, t =28. 115, P 〈0. 05 ]. CYP2W1 protein expression in the gastric cancer cell lines is higher than that in normal gastric mucosa cell lines [ （0. 481±0. 024） vs. 0, t = 49. 097, P 〈 0. 05 ]. The growth capacity of CYP2W1 positive gastric cancer cell is stronger than that of CYP2W1 negative cells （ P 〈 0. 05 ） , and CYP2WI positive gastric cancer cells are also more of invasiveness, [ （63±8 ） vs. （18±3 ） , t = 24. 134, P 〈0.05]. Conclusions CYP2W1 is only expressed in the gastric cancer tissues, hence it is closely related to the growth multiplication, and invasiveness of gastric cancer cells.

关 键 词：胃肿瘤 细胞增殖 肿瘤侵润 

分 类 号：R735.2[医药卫生—肿瘤]