低氧对大鼠前额叶皮层AQP4蛋白和HIF-1α蛋白表达的影响  

Effect of Hypoxia on Expression of AQP4 and HIF-1α in Protein in Prefrontal Cortex of Rats

作　　者：李敏[1] 董子玉[1] 陈学群[1] 

机构地区：[1]信阳职业技术学院,河南信阳464000

出　　处：《河南科技大学学报（医学版）》2016年第3期174-176,共3页Journal of Henan University of Science & Technology:Medical Science

摘　　要：目的探讨在低氧条件下SD大鼠前额叶皮层AQP4蛋白和HIF-1α蛋白的表达,为阐明低氧脑水肿的发病机制提供依据。方法将12只SD大鼠随机分为常氧组和低氧组,低氧组大鼠在低氧舱中模拟高原海拔7 000 m低氧(7.8%O2)中暴露8 h,通过Western blot检测大鼠前额叶皮层AQP4蛋白和HIF-1α蛋白的表达。结果在海拔7 000 m低氧暴露8 h后,SD大鼠前额叶皮层AQP4蛋白和HIF-1α蛋白表达增多(P<0.05)。结论 SD大鼠前额叶皮层AQP4蛋白和HIF-1α蛋白在模拟高原海拔7 000 m低氧8 h后表达升高,提示HIF-1可能通过上调AQP4蛋白的表达参与了低氧脑水肿的发生。Objective To investigate the expression of AQP4 and HIF-1α protein in the prefrontal cortex of SD rats under hypoxic conditions for the basis of elucidating the mechanism of hypoxic brain edema.Methods Twelve SD rats were randomly divided into the normal oxygen group and the hypoxia group. Rats in hypoxia group were exposed to hypoxia（ 7. 8% O2,equal to 7 000 m altitude） in a hypobaric chamber for8 h. The expression of AQP4 and HIF-1α protein in rat prefrontal cortex were determined by Western blot.Results AQP4 and HIF-1α protein were enhanced in the hypoxic rat prefrontal cortex after exposure to7 000 m hypoxia for 8 h（ P〈0. 05） by Western blot. Conclusion The expression of AQP4 and HIF-1αprotein in the prefrontal cortex of SD rats were increased after 8 h hypoxia. It was showed that HIF-1 might contribute to hypoxic brain edema by increasing the expression of AQP4 protein.

关 键 词：低氧 脑水肿 水通道蛋白4 低氧诱导因子-1 

分 类 号：R364.4[医药卫生—病理学] R338.25[医药卫生—基础医学]