中药类光敏剂金丝桃素的光谱学特性  

Spectral characteristic of traditional Chinese medicine photosensitizer hypericin

作　　者：王晓芳[1] 张昌莘[1] 林梓毫 方运良[1] 李天乐[1] 朱伟玲[1] 

机构地区：[1]广东石油化工学院理学院,广东茂名525000

出　　处：《中国医学物理学杂志》2016年第9期948-951,共4页Chinese Journal of Medical Physics

基　　金：国家自然科学基金(61475195);广东省自然科学基金(2015A030313873);广东省科技计划项目(2010b080701066)

摘　　要：本研究系统地研究了金丝桃素(HY)的紫外-可见吸收光谱、光漂白和荧光光谱特性,为HY的临床应用提供一定的参考。实验测量了不同浓度HY-二甲基亚砜溶液的紫外-可见吸收光谱,结果显示HY在可见光范围内存在多个吸收峰,其中,594 nm处的吸收最强,这表明594 nm为HY光动力治疗的首选光源波长;HY的光漂白特性研究表明,HY的初始浓度越高,光漂白所需的时间越长;HY的荧光光谱研究表明,荧光光谱在可见光范围内也存在多峰结构,其中,643 nm处的荧光峰最强且半峰宽最窄,因此,在光动力诊断中,采用643 nm荧光发射能大大提高检测的灵敏度。The ultraviolet-visible absorption spectrum, photobleaching and fluorescence spectrum characteristics of hypericin （HY） were systematically studied to offer some reference data for the clinical application of HY. The ultraviolet-visible absorption spectrum of varied concentrations of HY in dimethyl sulfoxide （DMSO） solution is investigated. The result indicates hat HY has several absorption peaks in the range of visible light, and that the absorption at 594 nm is maximum, which indicates that the 594 nm is the first choice for the HY photodynamic therapy. Photobleaching experiment results show that the higher the initial concentration of HY, the longer the time is required for the photobleaching. The fluorescence spectrum research shows that fluorescence spectrum has several peaks in the range of visible light, in which the fluorescence peak at 643 nm has the largest emission intensity and the lowest half-peak width. The detection sensitivity is greatly improved by using the fluorescence emission at 643 nm.

关 键 词：金丝桃素 光谱特性 吸收光谱 光漂白特性 荧光光谱 

分 类 号：R73[医药卫生—肿瘤] O43[医药卫生—临床医学]