茶多酚对直链烷基苯磺酸钠致小鼠皮肤损伤的保护作用  

The Protective Effects of Tea Polyphenol on Injury of Skin Tissue of Mice Induced by Linear Alkylbenzene Sulfonate

作　　者：赵文红[1] 崔慧娴[1] 孙倩男 陈兵兵[1] ZHAO Wenhong CUI Huixian SUN Qiannan CHEN Bingbing(Department of Preventive Medicine, Bengbu Medical College, Bengbu 233030, Chin)

机构地区：[1]蚌埠医学院预防医学系,安徽蚌埠233030

出　　处：《茶叶科学》2016年第5期461-468,共8页Journal of Tea Science

基　　金：安徽省高等学校自然科学重点研究项目(KJ2014A159);安徽省高等学校省级质量工程教学研究项目(2014jyxm210);安徽省高等学校省级质量工程特色专业建设项目(2014tszy019)

摘　　要：本研究主要探讨茶多酚(Tea polyphenol,TP)对直链烷基苯磺酸钠(Linear alkylbenzene sulfonate,LAS)引起小鼠皮肤组织损伤的保护作用。将50只健康昆明种雄性小鼠随机分为5组,每组10只,分别为正常对照组、TP对照组(TP 100 mg·L^(-1))、LAS损伤组(LAS 300 mg·L^(-1))、TP干预组1(LAS 300 mg·L^(-1),TP 100 mg·L^(-1))与干预组2(LAS 300 mg·L^(-1),TP 200 mg·L^(-1))。分别用蒸馏水及不同剂量的LAS及TP涂抹小鼠的背部剃毛处,连续涂抹60 d,观察各组小鼠的饮食、日常活动及体质量变化情况;取背部涂抹皮肤进行HE及Masson染色观察皮肤组织结构及胶原纤维变化情况;检测皮肤中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、羟脯氨酸(Hyp)及小鼠血清中乳酸脱氢酶(LDH)、一氧化氮(NO)水平。结果表明,TP干预可拮抗LAS引起的小鼠皮肤组织氧化应激及胶原改变;从第6周开始,干预组小鼠体质量增长显著高于LAS损伤组(P<0.01);皮肤形态结构有所改善,表皮厚度增加且较为完整,胶原纤维含量增加,纤维排列杂乱、松散、模糊的现象得到改善;皮肤中SOD、GSH-Px及Hyp水平明显增加,MDA含量显著减少(P<0.01);血清中LDH、NO水平显著低于LAS损伤组(P<0.01)。实验结果表明TP在一定程度上对LAS所致小鼠皮肤的损伤有保护作用。The objective of the study is to investigate the protective effects of tea polyphenol （TP） on injury of skin tissue of mice induced by linear alkylbenzene sulfonate （LAS）. Fifty healthy Kunming mice （male） were randomly divided into 5 groups including normal control group （NC）, TP control group （TPC）, LAS-injury group （LAS, 300 mg·L^-1）, TP-intervention group 1 （LAS 300 mg·L^-1, TP 100 mg·L^-1） and TP-intervention group 2 （LAS 300 mg·L^-1, TP 200 mg-L＂1） respectively （n=10）. The skins on the back of all mice were shaved and treated with distilled water and different concentrations of LAS and TP for 60 days. The observational indexes included general condition of mice , HE and Masson staining of skin, the levels of malondialdehyde （MDA）, super oxidase dimutase （SOD）, glutathione peroxidase （GSH-Px）, hydroxyproline （Hyp）, lactate dehydrogenase （LDH） and nitric oxide （NO） in skin and serum. The results showed that administration of TP to LAS-treated mice inhibited the oxidative stress and destruction of collagen fiber of skin induced by LAS. Compared with LAS-injury group, the body weight and skin thickness of mice of TP-intervention groups were obviously increased after treatment for 6 weeks （P〈0.01）. Additionally, the contents of collagen fibers were increased and the structure of collagen fibers was improved. Moreover, the levels of SOD, GSH-Px and Hyp were significantly increased, but the levels of MDA, LDH and NO were remarkably reduced （P〈0.01）. These results suggest that TP can protect skin of mice from LAS-induced injury.

关 键 词：茶多酚 直链烷基苯磺酸钠 皮肤组织 损伤 保护作用 

分 类 号：R285.5[医药卫生—中药学]