miR-221在前列腺癌细胞中的表达及对增殖的影响  

Effect of miR-221 expression on proliferation in prostate cancer cells

作　　者：毕学成[1] 刘久敏[1] 冯自卫[1] 吴永定 

机构地区：[1]广东省人民医院泌尿外科,广州510080 [2]广州医科大学公共卫生学院,广州510180

出　　处：《广州医药》2016年第5期1-4,共4页Guangzhou Medical Journal

基　　金：广东省医学科学技术研究基金项目(A2014016)

摘　　要：目的研究前列腺癌细胞中miR-221的表达情况及其对癌细胞增殖的影响。方法运用实时荧光定量PCR(qRT-PCR)检测miR-221在前列腺正常细胞株与前列腺癌细胞株中表达的差异情况,利用细胞转染构建miR-221过表达LNCa P和DU145细胞株,再通过CCK8细胞增殖实验检测细胞增殖情况的变化。结果 qRT-PCR检测细胞株发现miR-221在PC3、LNCa P和DU145三种前列腺癌细胞株中表达量均比前列腺正常细胞株Pr EC低(F=254.197,P<0.001),其中两两比较差异也均有统计学意义。细胞转染技术构建的miR-221过表达LNCa P和DU145细胞株,经qRT-PCR结果显示,miR-221在LNCa P和DU145细胞株中的表达水平明显升高(LNCa P,倍数变化=2.24,t=3.46,P<0.01;Du145,倍数变化=2.24,t=4.29,P<0.01)。细胞增殖实验结果显示,过表达了miR-221的LNCa P(P<0.001)和DU145(P<0.001)细胞生长速度慢于对照组。结论实验证明miR-221表达过度能减慢前列腺癌细胞的增殖,miR-221有可能成为前列腺肿瘤治疗的生物学标志物。Objective To investigate miR-221 expression in prostate cancer cells and its influence on prostate cancer cell proliferation. Methods miR-221 expressions in prostate normal cell lines and cancer cell lines were measured by qRTPCR. Overexpression of the miR-221 in LNCa P and DU145 cell lines used by cell transfection. Effects of the depletion on cell proliferation were assessed in vitro with CCK8. Results qRT-PCR showed miR-221 was lower expressed in PC3,LNCa P and DU145 than in Pr EC（ F = 254. 197,P〈 0. 001）,in which pairwise comparison also had significant differences. qRT-PCR showed miR-221 expression rose significantly in LNCa P and DU145 cell lines whose miR-221 was overexpression with cell transfection（ LNCa P,Fold Change = 2. 24,t = 3. 46,P〈 0. 001; Du145,Fold Change = 2. 24,t = 4. 29,P〈 0. 001）. Cell proliferation assay showed that growth of LNCa P（ P〈 0. 001） and DU145（ P〈 0. 001） cells whose miR-221 was overexpression was slower than the control group. Conclusion This study demonstrates miR-221 overexpression can inhibited the proliferation of prostate cancer cells for the first time,it also suggests that miR-221 has the potential to serve as a biomarker for PCa therapy.

关 键 词：前列腺癌 MIR-221 细胞增殖 

分 类 号：R737.25[医药卫生—肿瘤]