金银花尺蠖蛹粗多糖体外抗氧化活性  

Antioxidant activity of raw polysaccharides from Heterolocha jinyinhuaphaga pupae in vitro

作　　者：向玉勇[1] 章志坚[1] 殷培峰[1] 张元昶[1] 

机构地区：[1]滁州学院生物与食品工程学院,安徽滁州239000

出　　处：《浙江农林大学学报》2016年第5期862-868,共7页Journal of Zhejiang A&F University

基　　金：安徽高等学校自然科学研究项目(KJ2012B123);安徽省高等学校优秀青年人才基金资助项目(2009SQRZ147);国家级大学生创新创业训练计划项目(201210377006)

摘　　要：为了解金银花尺蠖Heterolocha jinyinhuaphaga蛹粗多糖的抗氧化活性,采用热水浸提法提取了金银花尺蠖蛹粗多糖,并测定其体外抗氧化活性。结果表明:金银花尺蠖蛹粗多糖对二苯代苦味酰自由基(DPPH),羟基自由基(·OH)以及超氧阴离子自由基(O2-·)具有一定的清除作用,在其质量浓度为0.2 g·L-1时的清除率分别为39.32%,35.62%和26.75%。随着多糖质量浓度的增大,对自由基的清除率也逐渐增加,当质量浓度上升到1.2 g·L-1时,清除率分别为74.93%,61.59%和47.84%,分别增加了35.61%,25.97%和21.09%,差异达显著水平(P<0.05)。在0.2~1.2 g·L-1的质量浓度范围内,清除率与粗多糖质量浓度之间呈显著的线性关系。金银花尺蠖蛹粗多糖具有较强的体外抗氧化活性,具有开发为抗氧化剂的潜力。To understand the antioxidant activity of raw polysaccharides from the Heterolocha jinyinhuaphaga pupae, and provide scientific reference for development of natural antioxidant drugs and performance foods, raw polysaccharides from the pupae was extracted in the laboratory by the hot water extraction method. Then, its antioxidant activity was studied in vitro by spectrophotometric method. Its clearing rate on 2,2-diphenyl-1-picrylhydrazyl(DPPH), ·OH and O_2^-. was tested with 3 replications, and vitamin C was used as control. Results showed that with a dosage of 0.2 g·L-1, the clearing rate of raw polysaccharide was 39.3% on DPPH,35.6% on ·OH, and 26.8% on O_2^-. With a 1.2 g·L-1dosage, the clearing rate increased to 74.9% for DPPH,61.6% for ·OH, and 47.8% for O_2^-. From 0.2 to 1.2 g·L-1, the clearing rate showed a linear connection to the dosages. Thus, the pupae polysaccharide of H. jinyinhuaphaga had a high antioxidant activity, which could potentially be exploited.

关 键 词：昆虫学 金银花尺蠖 蛹粗多糖 自由基 抗氧化活性 

分 类 号：S763.42[农业科学—森林保护学] Q964[农业科学—林学]