SH-SY5Y神经细胞中α7神经型尼古丁受体基因过表达对CaMK Ⅱ和CREB的影响  

Effects of α7 neuronal nicotinic receptor gene overexpression in SH-SY5Y cells on the levels of CaMK Ⅱ and CREB

作　　者：吕园园[1] 张淑丽[1] 吴昌学[1] 王晓玲[1] 官志忠[1,2] 齐晓岚[1] 

机构地区：[1]贵州医科大学分子生物学重点实验室,贵州贵阳550004 [2]贵州医科大学病理学教研室,贵州贵阳550004

出　　处：《中风与神经疾病杂志》2016年第9期772-775,共4页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金资助项目(81360178);教育部"长江学者和创新团队发展计划资助"(IRT13058);贵州省科技厅重大专项(黔科合重大专项字2014[6008]);贵州省科技厅项目(201344;黔科合SY字[2013]3020)

摘　　要：目的研究SH-SY5Y神经细胞中α7 nAChR基因过表达对CaMKⅡ和CREB的影响。方法复苏稳定转染α7 nAChR-pc DNA3.1质粒及空载质粒的SH-SY5Y神经细胞后,用含G418的培养液进行筛选培养;应用实时荧光定量PCR法和蛋白印迹法(Western blot)检测α7 nAChR基因过表达组、空载质粒组和正常对照组细胞中CaMKⅡ、CREB mRNA及蛋白表达水平的变化。结果与对照组相比,α7 nAChR基因过表达细胞组的CaMKⅡ、CREB mRNA表达水平分别增加了116.8%和114.7%(P<0.01);及CaMKⅡ、CREB蛋白表达水平分别增加了8.7%(P>0.05)和41.4%(P<0.05)。结论α7 nAChR的神经保护作用可能与上调细胞CaMKⅡ、CREB水平有关。b Objective To study the effects of α7 nAChR gene overexpression on the levels of CaMKⅡand CREB in SH-SY5Y cells.Method SH-SY5Y cells were transfected with α7 nAChR-pcDNA3.1 plasmid,the stable clones were cul-tivated with culture medium containing G418.Real-time PCR and Western blot were used to detect the mRNA and protein levels of CaMKⅡand CREB,respectively;Results Compared to negative controls and controls,SH-SY5Y with α7 nAChR gene overexpression increased the mRNA levels of CaMKⅡand CREB by 116.8% and 114.7%,respectively;increased the protein levels of CaMKⅡand CREB by 8.7% and 41.4%,respectively.Conclusion Neuroprotective effect of α7 nAChR may be related to up-regulation of CaMKⅡand CREB levels.

关 键 词：α7 NACHR SH-SY5Y细胞 CaMKⅡ CREB 

分 类 号：Q51[生物学—生物化学] R749.16[医药卫生—神经病学与精神病学]