UPLC测定多伞阿魏中倍半萜烯香豆素(DAW22)的含量  被引量:3

Content Determination of Sesquiterpene Coumarin( DAW22) in Ferula ferulaeoides by UPLC

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作  者:朱芸[1] 张珂[1] 梁旭[1] 谭勇[1] 黄健[2] 王金辉[1] 

机构地区:[1]石河子大学药学院新疆特种植物药资源教育部重点实验室,新疆石河子832002 [2]沈阳药科大学中药学院,沈阳110016

出  处:《中国实验方剂学杂志》2016年第19期63-65,共3页Chinese Journal of Experimental Traditional Medical Formulae

基  金:国家科技支撑计划项目(2012BAI30B02);国家自然科学基金项目(81460574;81303270);石河子大学高层次科研启动基金项目(RCZX201440);石河子大学应用基础研究青年项目(2015ZRKXYQ16)

摘  要:目的:利用超高效液相色谱法测定了分布于新疆的不同生长时期多伞阿魏中DAW22的含量。方法:采用固相萃取(SPE)净化,测定条件Waters ACQUITY UPLC BEH C18色谱柱(2.1 mm×50 mm,1.7μm),以甲醇-1%甲酸水溶液为流动相,进行梯度洗脱,流速0.2 m L·m L^(-1),柱温30℃,波长316 nm,进行含量测定。结果:DAW22进样量在6.21~124.2 ng(r=1.000 0)与其峰曲线下面积呈良好的线性关系,平均回收率99.81%,RSD 2.0%,生长在5月9日的多伞阿魏植株中DAW22的含量最高。结论:建立的多伞阿魏中DAW22的质量控制研究方法准确、可靠、重复性好,能够用于多伞阿魏的质量控制。Objective: To establish UPLC method for the determination of DAW22 from Ferula ferulaeoides of different growth periods in Xinjiang. Method: Solid phase extraction( SPE) was used for purification,and the determination was performed on ACQUITY UPLC BEH C18( 2. 1 mm × 50 mm,1. 7 μm)with methanol-1% formic acid solution as the mobile phase for gradient elution. The flow rate was 0. 2 m L·min-1.Column temperature was 30 ℃ and UV detection wavelength was 316 nm. Result: The sample volume showed good linear relationship with area under the peak curve within the range of 6. 21-124. 2 ng( r = 1. 000 0) for DAW22.The average recovery was 99. 81%,and the RSD was 2. 0%. The content of DAW22 was highest for those grown in May 9th. Conclusion: This method is accurate,simple and reproducible,and can be applied to the quality control of F. ferulaeoides.

关 键 词:多伞阿魏 倍半萜烯香豆素 超高效液相色谱 含量测定 

分 类 号:R284.1[医药卫生—中药学]

 

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