紫杉醇脂质体对兔舌癌淋巴结转移癌细胞凋亡的研究  

The effect of paclitaxel liposome on the apoposis of cancer cells in the lymph node with the metastasis of rabbit tongue cancer

作　　者：张立刚[1] 黄桂林[1] 胡小华[1] 张霓霓[1] 易杰[1] 姚礼[1] 刘泽念 

机构地区：[1]遵义医学院附属口腔医院,563003 [2]遵义医药高等专科学校

出　　处：《实用口腔医学杂志》2016年第5期607-610,共4页Journal of Practical Stomatology

基　　金：贵州研究生工作站项目(编号:黔教研合YJSZ字[2013]08);教育部留学回国人员科研启动基金资助项目(编号:教外司留[2011]508号);遵义医学院硕士启动基金(编号:2012 F-618);遵义医学院附属口腔医院院内基金(编号:KY2015-12);遵义市红花岗区科学技术局项目(编号:遵红科合社字(2015)09号)

摘　　要：目的:研究紫杉醇对兔舌VX-2移植性鳞癌颈部转移淋巴结内癌细胞凋亡的影响。方法:24只兔舌VX-2癌颈淋巴结转移模型随机选择舌动脉或耳缘静脉分别灌注紫杉醇脂质体、紫杉醇注射液或5%葡萄糖溶液。化疗后7 d收集转移淋巴结,透射电镜观察凋亡细胞超微结构,流式细胞仪检测癌细胞凋亡率,SP免疫组化检测癌组织P53蛋白表达。结果:动脉给药组细胞凋亡率高于静脉给药组,脂质体组高于游离药物组(P<0.05)。动脉灌注紫杉醇脂质体组P53蛋白阳性率最低(P<0.05)。结论:紫杉醇脂质体诱导淋巴结转移癌细胞凋亡作用较游离紫杉醇强,区域性动脉灌注较全身静脉灌注更有效。Objective： To study the effect of paclitaxel liposome on the apoptosis of cancer cells in neck metastatic lymph node of rabbit tongue cancer. Methods： 24 New Zealand rabbits with VX-2 tumor at tongue edge were divided into 6 groups randomly after neck lymph node metastasis. Paclitaxel liposome, free paclitaxel and 5% glucose were injected into the lingual arterial and ear marginal vein, respectively. A week after chemotherapy metastasis lymph nodes were collected. The apoptosis of the cancer cells in the lymph nodes was examined by transmission electron microscope, flow cytometry, and SP immunohistochemical method for P53 protein expression of the cancer tissue. Results： The apoptosis rate of cancer cells in the intra-arterial chemotherapy group was higher than that in the intra-venous chemotherapy group, in the paclitaxel liposome injection group was higher than that in the free paclitaxel injec- tion group（ P 〈 0.05 ）. The positive rates of P53 protein expression in paclitaxel liposome group by intra-arterial injection was the lowest（ P 〈0.05 ）. Conclusion： Paclitaxel liposome is more effective than free paclitaxel, regional arterial infusion is more effective than intra-venous infusion in the apoptosis induction of lymph node metastasis cancer cells.

关 键 词：紫杉醇脂质体 游离紫杉醇 细胞凋亡 靶动脉灌注 区域淋巴结 

分 类 号：R739.86[医药卫生—肿瘤]