cAMP信号通路在根尖牙乳头干细胞定向分化中的作用  

The role of cAMP signaling on the differentiation of stem cells from the apical papilla

作　　者：苏圣哲 朱永娜[1] 张菁[1] 李颂[1] 

机构地区：[1]安徽医科大学口腔医学院,安徽医科大学附属口腔医院,安徽省口腔疾病研究中心实验室,合肥230032

出　　处：《实用口腔医学杂志》2016年第5期650-654,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:81400497);安徽省自然科学基金(编号:1408085QH178)

摘　　要：目的:探讨环磷酸腺苷(c AMP)信号通路对根尖牙乳头干细胞(SCAP)定向分化的影响。方法:酶消化法培养细胞;在矿化诱导液中分别加入信号通路激活剂Forskolin(FOR)和抑制剂H-89;通过茜素红染色和定量分析检测钙盐沉积;QPCR检测相关矿化基因m RNA的表达。结果:激活SCAPs内的c AMP信号后,该细胞茜素红染色增强,并上调矿化基因ALP、OCN、OSX、RUNX2的m RNA表达;而抑制SCAPs中c AMP信号后,该细胞茜素红染色减弱,并下调矿化基因的表达。结论:激活c AMP/PKA信号通路促进SCAPs分化,抑制信号通路则发挥相反作用,说明该信号通路对根尖牙乳头干细胞向成牙/成骨分化有一定的调节作用。Objective： The aim of this study was to investigate the role of cyclic adenosine monophosphate（cAMP） signaling on the differentiation of stem cells from the apical papilla （SCAPs）. Methods： SCAPs were cultured by enzyme digestion method. After adding activator （Forskolin） and inhibitor （H-89） into the culture of SCAPs in mineralization-inducing medium, calcium deposition was measured by alizarin red staining. QPCR was performed to measure the mRNA expression of mineralization-related genes. Results： When the cAMP signaling was activated, calcium deposition and the mRNA expression of ALP, OCN, OSX and RUNX2 increased sharply. After inhibiting the signaling, calcium deposition and the mRNA expression of ALP, OCN, OSX and RUNX2 decreased. Conclusion： The cAMP signaling may promote the odontogenic and osteogenic differentiation of SCAPs.

关 键 词：根尖牙乳头干细胞(SCAPs) CAMP 分化 

分 类 号：R781[医药卫生—口腔医学]