microRNA-29c过表达对人胰腺癌AsPC-1、PANC-1细胞增殖的影响  

Effect of microRNA-29c Over-expression on Proliferation of Human Pancreatic Cancer Cells

作　　者：周显飞 田舍[1] 王杰[1] 贾亮亮[1] 喻超[1] 江建新[1] 

机构地区：[1]贵州医科大学附院肝胆外科,贵州贵阳550004

出　　处：《贵阳医学院学报》2016年第9期1002-1005,共4页Journal of Guiyang Medical College

基　　金：国家国际科技合作专项资助(2014DFA31420);国家自然科学基金资助项目(81160311)

摘　　要：目的:探讨microRNA-29c过表达对人胰腺癌As PC-1、PANC-1细胞增殖的影响。方法:构建含microRNA-29c过表达腺病毒并感染至胰腺癌As PC-1及PANC-1细胞(实验组),感染空载体作为阴性对照组,采用实时定量逆转录聚合酶链反应(qRT-PCR)检测As PC-1和PANC-1感染microRNA-29c 24 h时的感染效率,CCK-8实验检测microRNA-29c感染48 h时人胰腺癌细胞的增殖能力,细胞平板克隆实验检测感染microRNA-29c24 h人胰腺癌细胞的单克隆形成能力。结果:与阴性对照组比较,microRNA-29c感染As PC-1及PANC-1细胞后microRNA-29c表达水平明显升高;过表达microRNA-29c后As PC-1和PANC-1细胞增殖能力明显受到抑制,细胞的克隆形成数明显减少(P<0.01)。结论:microRNA-29c过表达能有效抑制胰腺癌细胞的增殖能力,有望成为治疗胰腺癌的新靶标。Objective： To investigate the effect of miR-29c over-expression on the proliferation of hu- man pancreatic cancer cells, AsPC-1 ,PANC-1. Methods： The recombination adenovirus Ad-miR-29c and control adenovirus was constructed and transfected human pancreatic cancer cells, AsPC-1 and PANC-1 cell （experiment group）. The expressions of miR-29c in AsPC-1 and PANC-1 cells were de- tected by real-time PCR. The CCK-8 assay was applied to examine the proliferation ability of miR-29c on the proliferation of human pancreatic cancer cell. The cell colony formation assay was used to meas- ure the growth of the cells after infected by miR-29c for 24 h. Results： After infected with Ad-miR- 29c and PANC-1, the miR-29c expression levels increased;the over-expression of microRNA-29c in the pancreatic cancer cells significantly inhibited the proliferation abilities of AsPC-1 and PANC-1 ceils, cell clone formation abilities were also significantly decreased （P 〈0.01 ）. Conclusion： miR- 29c can effectively suppress the proliferation of human pancreatic cancer cells, which makes a promis- ing new therapeutic target for pancreatic cancer.

关 键 词：微小RNA microRNA-29c 胰腺癌 细胞增殖 腺病毒 

分 类 号：R735.9[医药卫生—肿瘤]