莲黄子复方对裸鼠膀胱癌T24细胞移植瘤的抑制作用  

Inhibitory Effect of Lian-huang zi Compound on Human Bladder Cancer T24 Cells Xenografted Tumor in Nude Mice

作　　者：任瑞锋[1] 彭艳军[1] 罗英[1] 赵文艳[1] 赵连梅[2] 单保恩[2] 

机构地区：[1]华北油田总医院,河北任丘062552 [2]河北医科大学第四医院,河北石家庄050011

出　　处：《贵阳医学院学报》2016年第9期1042-1046,1067,共6页Journal of Guiyang Medical College

基　　金：河北省自然科学基金课题(H2015206376)

摘　　要：目的:研究莲黄子(半边莲、大黄、五加皮、决明子)复方对膀胱癌T24细胞裸鼠移植瘤的抑瘤作用。方法:Nu/Nu裸鼠接种T24细胞构建膀胱癌T24细胞移植瘤模型,15只模型裸鼠随机分为莲黄子复方低剂量治疗组、高剂量治疗组和对照组,每组5只;治疗组裸鼠分别予2.5 m L/kg或5 m L/kg莲黄子复方药液灌胃治疗,对照组予5 m L/kg生理盐水灌胃,治疗当天定义为0天;其后,隔日灌胃,总计7次;治疗后1天开始隔日测量裸鼠移植瘤的体积,末次治疗后第2天处死裸鼠,称量瘤体重量;采用ELISA法检测裸鼠血清肿瘤坏死因子(TNF)-α、粒-巨噬细胞集落刺激因子(GM-CSF)、γ-干扰素(IFN-γ)含量;免疫组织化学法检测裸鼠移植瘤组织凋亡相关蛋白Bcl2和Ba X及抑癌基因PTEN的表达。结果:治疗前各组模型裸鼠移植瘤体积比较,差异无统计学意义(P>0.05);治疗结束时,两种剂量治疗组裸鼠的肿瘤体积和重量均明显小于对照组(P<0.05);同时,相比对照组,两种剂量治疗组裸鼠血清TNF-α含量均明显升高,而GM-CSF、IFN-γ含量则明显降低(P<0.05);移植瘤组织中Bcl2表达在高剂量治疗组、低剂量治疗组及对照组间呈现递增的趋势,而Bax和PTEN表达则呈递减趋势,且组间差异均有统计学意义(P<0.05)。结论:莲黄子配方对膀胱癌T24细胞裸鼠移植瘤有明显的抑制作用,其机制可能与其诱导PTEN和Bax的表达及抑制Bcl2的表达有关。Objective： To investigate the inhibitory der cancer T24 cells xenografted tumor in nude mice effects of lian-huang zi compound to human blad- Methods： The Nu/Nu nude mice model of xen- ografted tumor was established by injection of human bladder cancer T24 cells. Fifteen model mice were randomly divided into 3 groups： low dose lian-huang zi compound treatment group, high dose lian-huang zi compound treatment group, and control group. Mice in treatment group received gastric feeding of 2.5 mL/kg lian-huang zi compound （low dose group） , 5 mL/kg lian-huang zi compound （high dose group） while mice in control group received gastric feeding of 5 mL/kg 0.9% sodium chlo- ride. The first administration day was termed as day 0. Then, mice received gastric feeding once every two days and totally received 7 times gastric feeding. The tumor volume of mice was measured once every two days from 1 day after administration. The mice were sacrificed after the next day of last admin- istration, and the mice weight and the tumor weight were measured. The serum of mice in each group was collected, and the expression of TNF-α, GM-CSF and IFN-γ were analyzed by EL1SA. The ex- pression of apoptosis associated protein bcl-2 and bax, associated antioncogene PENT in xenografted tumor were detected by immunocytochemistry. Results： There were no difference between the size of xenografted tumor in each group before administration （p 〉 0.05）. After treatment, the size or weight of xenografted tumor in the 2 treatment groups were significantly decreased compared with control group （P 〈 0.05）. Compared with control group, the content of serum TNF-α significantly increased in the treatment group （P 〈 0.05 ） while the content of serum GM-CSF and IFN-γ significantly decreased （P 〈 0.05 ）. The expression of apoptosis associated protein Bcl-2 in xenografted tumor increased gradually from the high dose treatment group to the low dose treatment group and control group （ P 〈 0.05 ）. The expression

关 键 词：莲黄子配方 膀胱癌 T24细胞 抑瘤率 模型 细胞凋亡 

分 类 号：R737.14[医药卫生—肿瘤]