NSAIDs肠病大鼠回肠SIgA、IL-1β、IL-8、IL-10水平及树突状细胞数的变化  

Changs of SIg A,IL-1β、IL-8、IL-10 Levels and Dendritic Cells in Intestinal Mucosa of Rats with NSAIDs Enteropathy

作　　者：肖源[1] 车筑平[2] 程华[1] 杨红静[3] 谭庆华[4] 

机构地区：[1]贵州医科大学第三附属医院消化内科,贵州都匀558000 [2]贵州医科大学附院内镜中心,贵州贵阳550004 [3]贵州医科大学临床医学院,贵州贵阳550004 [4]四川大学华西医院消化内科,四川成都610041

出　　处：《贵阳医学院学报》2016年第9期1064-1067,共4页Journal of Guiyang Medical College

基　　金：贵州省科技厅基金(E2011-10)

摘　　要：目的:探讨非甾体类消炎药(NSAIDs)肠病时SD大鼠回肠分泌型免疫球蛋白A(SIg A)、白细胞介素-1β(IL-1β)、IL-8、IL-10水平及树突状细胞数的变化。方法:34只清洁级SD大鼠均分为药物损伤模型组(模型组)和对照组,模型组大鼠腹腔注射阿司匹林(100 mg/kg,2次/d),对照组腹腔注射等量生理盐水;造模2周后处死大鼠,在距回盲瓣5 cm近端肠管切取回肠段2 cm,采用ELISA法检测大鼠回肠黏膜组织SIg A、IL-1β、IL-8及IL-10水平,免疫组织化学(IHC)检测回肠黏膜CD205阳性染色的树突状细胞数量。结果:HE染色结果显示,模型组黏膜上皮细胞破坏,黏膜杯状细胞减少,隐窝结构变形,固有腺体排列紊乱,黏膜以及黏膜下层见大量淋巴细胞以及单核细胞;对照组黏膜正常,未被破坏;免疫组织化学染色后,与对照组比较,模型组大鼠CD205阳性细胞增多,IOD值升高(P<0.05);模型组大鼠回肠黏膜SIg A降低(P<0.05),IL-1β、IL-8及IL-10增加,树突状细胞数量增多,差异有统计学意义(P<0.05)。结论:NSAIDs肠病发生后,回肠黏膜保护屏障受损,体液免疫紊乱。Objective ： To investigate the changes of non-steroidal anti-inflammatory drug enteropathy in SD rats with ileal SIgA, interleukin-1β, interleukin-8, interleukin-10 and dendritic cells change. Methods： 34 SPF grade Sprague-Dawley rats were randomly divided into 2 groups in equal number. The aspirin group received intraperitoneally injections of 100 mg/kg of aspirin twice a day for 14 days, the control group received equal volume of normal saline. 5 cm from the ileocecal valve in the proximal intestine was cut off by 2 cm after constructing the model for 2 weeks and executed the rats. The levels of SIgA, IL-1β、IL-8、IL-10 in ileal tissue were detected by ELISA. The quantity of CD205-posi- tive cells in mueosa was tested by immunohistoehemistry analysis. Results： Aspirin group compared with control group, the level of SIgA in ileal tissue of aspirin group decreased （ P 〈 0.05 ）, IL-1β、IL-8、IL-10 and the number of dendritic cells increased （P 〈 0.05 ） ; mucosa of control group was normal and stayed intact; after IHC staining, CD205 positive cells increased in model rats, comparing with control group, observation showed increased IOD value（P 〈0.05） ; comparing with control group, SI- gA in model group rats ileal mucosa decreased（P 〈 0.05） , IL-1β、IL-8、IL-10 increased, dendrit- ic cells increased （ P 〈 0.05 ）. Conclusion： NSAIDs bowel disease would damage intestinal mucosal barrier and caused thumoral immune disorder.

关 键 词：树突状细胞 分泌型免疫球蛋白A 白细胞介素 非甾体类消炎药肠病 大鼠 Sprague-Dawley 

分 类 号：R574[医药卫生—消化系统]