灯盏花发根培养条件筛选研究  

Establishment of Hairy Root Culture of Erigeron breviscapus

作　　者：黄鑫[1,2] 季倩[2] 陈瑞兵[2] 何德[1] 张磊 

机构地区：[1]西南林业大学生命科学学院,云南昆明650224 [2]第二军医大学药学院,上海200433

出　　处：《西南林业大学学报（自然科学）》2016年第5期21-26,共6页Journal of Southwest Forestry University：Natural Sciences

摘　　要：为建立灯盏花的发根培养体系,用C58C1发根农杆菌空菌株和携带目的基因的菌株转化灯盏花叶片获得发根,测定发根的生长曲线,比较不同因素对发根生长量的影响。结果表明:利用发根农杆菌C58C1菌株成功从灯盏花中诱导出了发根,并建立了灯盏花发根最优的培养体系,其诱导的最佳农杆菌菌液浓度为OD_(600)=0.6,最佳浸染时间为10 min,最佳共培养时间为2 d,在此条件下诱导率高达60%。经PCR鉴定证明Ri质粒的T-DNA已成功转化灯盏花的发根。To establish the cultural system for hairy root of Erigeron breviscapus root of E. breviscapus was ob- tained from leaf explants after infected with Agrobacterium rhizogenes C58C1 strains, determine the growth curve of hairy roots, and compared the effect of hairy roots increment by different factors. The results showed that the hairy root of E. breviscapus was successfully induced by using A. rhizogenes strain C58C1, and the best system of hairy root culture was established. The best concentration of A. rhizogenes liquid is OD600 = 0. 6, the best infection time is 10 min, and the best co-culture time is 2 d. These conditions allow the inducement rate was 60%. The transforma- tion of T-DNA from Ri plasmid to the hairy root was confirmed by PCR analysis.

关 键 词：发根农杆菌 诱导率 遗传转化体系 灯盏花 培养基 

分 类 号：S723.1[农业科学—林木遗传育种]