无籽刺梨多倍体诱导试验初报  

Preliminary Report on Poly Ploidy Induction of Rosa sterilis

作　　者：李斌[1,2] 林源 辛亚龙 唐军荣[1,2] 尹丽莎 韩国伟[1,2] 辛培尧[1,2] 

机构地区：[1]西南林业大学云南省高校林木遗传改良与繁育重点实验室,云南昆明650224 [2]西南林业大学国家林业局西南地区生物多样性保育重点实验室,云南昆明650224 [3]大理农林职业技术学院,云南大理671003

出　　处：《西南林业大学学报（自然科学）》2016年第5期27-31,共5页Journal of Southwest Forestry University：Natural Sciences

基　　金：云南省林学一级学科博士点建设项目;西南林业大学云南省高校林木遗传改良与繁育重点实验室开放基金项目;云南省省院省校教育合作咨询共建重点学科项目(211015)资助

摘　　要：采用育种技术对无籽刺梨进行多倍体诱导,以期获得无籽刺梨多倍体植株。以秋水仙素作为诱导剂,二倍体组培苗为材料,比较不同的预培养时间、处理时间及秋水仙素浓度对无籽刺梨染色体加倍的诱导效果。结果表明:无籽刺梨茎段在分化培养基上预培养1 d后,继而用含300 mg/L秋水仙素溶液浸泡处理12 h,再进行分化培养的诱导效果最佳,其诱导变异率达25.6%;无籽刺梨多倍性植株同质化培养的最佳次数为6次。对变异植株根尖细胞进行染色体计数后发现,部分变异植株的根尖细胞染色体为2n=4x=28,为四倍体。部分植株同时存在2n=2x=14和2n=4x=28两种倍性细胞,为嵌合体。The polyploidy breeding technology was used to induce Rosa sterilis polyploidy, for the obtaining of R. sterilis polyploidy plants. The colchicine was used as induction dose, diploid cuvette seedling of R. sterilis as material, to compare induction effect on chromosome doubling in different precuhure time, different colchicine con- centration and different treatment time. The results showed that after preculture in differentiation medium for 1 d, and soaking in 300 mg/L colchicine solution for 12 h, then differentiation culture in differentiation medium, the induction effect was better, and the mutation rate was 25.6%. The best culture frequency of homogenization was 6. After observing the chromosome number in root-tip of mutation plants, it was found that chromosome number in some tetraploid plants cells was 2n = 4x = 28, while cells with both 2n = 2x = 14 and 2n = 4x = 28 in some chimera plants.

关 键 词：无籽刺梨 多倍体诱导 浸泡处理 染色体数 组织培养 

分 类 号：S718.46[农业科学—林学]